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Investigations on anti-Aspergillus properties of bacterial products

  1. V. Yadav1,
  2. J. Gupta1,
  3. R. Mandhan3,
  4. A.K. Chhillar1,
  5. R. Dabur2,
  6. D.D. Singh2,
  7. G.L. Sharma2

Article first published online: 25 JUL 2005

DOI: 10.1111/j.1472-765X.2005.01772.x

Issue

Letters in Applied Microbiology

Letters in Applied Microbiology

Volume 41, Issue 4, pages 309–314, October 2005

Additional Information

How to Cite

Yadav, V., Gupta, J., Mandhan, R., Chhillar, A.K., Dabur, R., Singh, D.D. and Sharma, G.L. (2005), Investigations on anti-Aspergillus properties of bacterial products. Letters in Applied Microbiology, 41: 309–314. doi: 10.1111/j.1472-765X.2005.01772.x

Author Information

  1. 1

    Institute of Genomics and Integrative Biology, University Campus, Delhi

  2. 2

    Department of Biomedical Sciences, Bundelkhund University, Jhansi

  3. 3

    Department of Biotechnology, Kurukshetra University, Kurukshetra, India

*G.L. Sharma, Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi-110007, India (e-mail: drglsharma@hotmail.com).

Publication History

  1. Issue published online: 25 JUL 2005
  2. Article first published online: 25 JUL 2005
  3. 2005/0090: received 28 January 2005, revised 19 April 2005 and accepted 1 June 2005

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Keywords:

  • anti-Aspergillus;
  • antifungal protein;
  • Aspergillus;
  • bacterial products

Abstract

Aims:  To investigate the anti-Aspergillus properties of bacterial products.

Methods and Results:  In the present study, 12 bacterial strains were screened for antifungal activity against Aspergilli. The culture supernatant and lysates of Pseudomonas aeruginosa, Bacillus cereus, Escherichia coli (BL21, DH5α, HB101, XL Blue), Klebsiella pneumoniae, Streptomyces thermonitrificans, Streptococcus pneumoniae, Enterobacter aerogenes, Staphylococcus aureus and Salmonella typhi were examined for antifungal activity in protein concentration ranging from 1000·0 to 7·8 μg ml−1 using microbroth dilution assay. The lysate of Salm. typhi and E. coli BL21 exhibited the maximum activity against Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger. Their in vitro minimum inhibitory concentrations (MICs) were found to be 15·6–31·2 μg ml−1 by microbroth dilution and spore germination inhibition assays. In disc diffusion assay, a concentration of 3·1 μg disc−1 of Salm. typhi lysate showed significant activity against Aspergilli. Escherichia coli BL21 exhibited similar activity at 6·2 μg disc−1. The work on identification of molecule endowed with antimycotic properties is in progress.

Conclusion:  The products of Salm. typhi and E. coli demonstrated significant activity against Aspergillus species.

Significance and Impact of the Study:  This is the first time that E. coli has been reported for anti-Aspergillus activity. It could be an important source of biologically active compounds useful for developing better new antifungal drugs/or probiotics.

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