Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples
Article first published online: 17 JUL 2006
Letters in Applied Microbiology
Volume 43, Issue 4, pages 364–369, October 2006
How to Cite
Sweeney, F.P., Courtenay, O., Ul-Hassan, A., Hibberd, V., Reilly, L.A. and Wellington, E.M.H. (2006), Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples. Letters in Applied Microbiology, 43: 364–369. doi: 10.1111/j.1472-765X.2006.01983.x
- Issue published online: 17 JUL 2006
- Article first published online: 17 JUL 2006
- 2006/0202: received 14 February 2006, revised and accepted 15 May 2006
- bovine tuberculosis;
- immunomagnetic capture;
- Meles meles;
- Mycobacterium bovis
Aims: To adapt an immunomagnetic capture (IMC) technique to concentrate and cultivate Mycobacterium bovis from environmental samples including soil, faeces and urine.
Methods and Results: Cells of Myco. bovis BCG and wild-type Myco. bovis were successfully isolated and cultured from seeded and naturally infected materials respectively. The IMC cell recovery estimated by colony forming units (CFUs) counts ranged from 0·10% to 0·16% for spiked media, and 0·15–0·36% for naturally infected soil and faeces. Recovery estimated by cell counts calculated using semi-quantitative PCR ranged from 80·3% to 88·6% for spiked and 84·1–88·2% for naturally infected material. The differences in the recovery rates estimated by CFUs compared with pixel intensity is likely to be due to clustering of cells on culture plates, thereby underestimating the true cell count.
Conclusions: The IMC techniques can be applied to isolate viable wild type Myco. bovis from naturally contaminated environmental samples.
Significance and Impact of Study: Cultivation of Myco. bovis from environmental samples using traditional methods is extremely problematic. Here, we demonstrate a novel development of IMC techniques that will greatly facilitate the study of the organism in situ in order to assess its epidemiological importance in bovine tuberculosis persistence.