Present address Arthur C. Ouwehand, Danisco Innovation, FIN-02460 Kantvik, Finland.
Effect of starch- and lipid-based encapsulation on the culturability of two Bifidobacterium longum strains
Article first published online: 25 JAN 2007
Letters in Applied Microbiology
Volume 44, Issue 5, pages 500–505, May 2007
How to Cite
Lahtinen, S.J., Ouwehand, A.C., Salminen, S.J., Forssell, P. and Myllärinen, P. (2007), Effect of starch- and lipid-based encapsulation on the culturability of two Bifidobacterium longum strains. Letters in Applied Microbiology, 44: 500–505. doi: 10.1111/j.1472-765X.2007.02110.x
- Issue published online: 25 JAN 2007
- Article first published online: 25 JAN 2007
- 2006/1198: received 23 August 2006, revised 15 November 2006 and accepted 21 November 2006
Aims: To assess the applicability of starch- and lipid-based encapsulation methods for improving the viability and culturability of two Bifidobacterium longum strains stored in fermented and nonfermented foods.
Materials and Results: Cells were encapsulated with partially hydrolysed potato starch granules combined with amylose coating, or entrapped in cocoa butter matrix. The tested B. longum strains were not adherent to the starch granules, and the culturability of the cells stored in fermented and nonfermented foods was not improved by starch-based encapsulation. Encapsulation of the cells in cocoa butter was found to increase the plate counts during storage. In addition to plate counts, viability of the cells was measured by fluorescent microscopy using LIVE/DEAD BacLight viability assay. Microscopic counts of the viable cells did not change significantly during storage, suggesting that the cells remained alive despite becoming unable to grow on nutrient agar plates.
Conclusions: Encapsulation with cocoa butter increased the culturability of the cells, but encapsulation with hydrolysed potato starch had no effect. Culture-independent viability assay suggested that cells remained viable despite being unable to grow on agar plates.
Significance and the Impact of the Study: This study indicates that encapsulation techniques may be useful in improving the culturability of bacteria, but the plate counts may yield insufficient data on the actual viability of the cells.