Use of Hugh and Leifson’s medium as a simple screening test to aid in the differentiation of Arcobacter spp. from background flora during their isolation from foodstuffs
Article first published online: 22 AUG 2008
© 2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 47, Issue 3, pages 187–191, September 2008
How to Cite
Hamill, S., Neill, S.D. and Madden, R.H. (2008), Use of Hugh and Leifson’s medium as a simple screening test to aid in the differentiation of Arcobacter spp. from background flora during their isolation from foodstuffs. Letters in Applied Microbiology, 47: 187–191. doi: 10.1111/j.1472-765X.2008.02399.x
- Issue published online: 27 AUG 2008
- Article first published online: 22 AUG 2008
- 2007/1777: received 6 November 2007, revised 25 April 2008 and accepted 28 April 2008
Aims: To investigate the suitability of Hugh and Leifson’s medium (HLM) as the basis of a simple screening test to differentiate between contaminants and Arcobacter spp. during their isolation from foodstuffs.
Methods and Results: Characterized Arcobacter spp. were obtained from recognized culture collections. Wild-type isolates of Arcobacter spp. and contaminants were obtained using published isolation protocols. Retail packs of red meats were used as the source of the isolates. Eighteen defined Arcobacter spp. gave no reaction on HLM, as did 10 local wild-type isolates. Overall 163 contaminants were studied for oxidative reactions on HLM and 86% of isolates demonstrated this property.
Conclusions: HLM can usefully serve as a simple and effective screening test to differentiate between Arcobacter spp. and contaminants.
Significance and Impact of the Study: Arcobacter isolation procedures are still being developed, and no effective diagnostic media currently exist. Rapidly excluding most contaminants can markedly increase the efficiency of isolation procedures by removing the need for extensive biotyping or the requirement to isolate DNA and conduct PCR tests.