Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs
Version of Record online: 16 OCT 2008
© 2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 47, Issue 5, pages 367–373, November 2008
How to Cite
Guo, X., Xia, X., Tang, R., Zhou, J., Zhao, H. and Wang, K. (2008), Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Letters in Applied Microbiology, 47: 367–373. doi: 10.1111/j.1472-765X.2008.02408.x
- Issue online: 16 OCT 2008
- Version of Record online: 16 OCT 2008
- 2008/0203: received 5 February 2008, revised 27 April 2008 and accepted 30 April 2008
- lean pigs;
- obese pigs;
- real-time PCR
Aims: To investigate whether the relative abundance of the Bacteroidetes and Firmicutes divisions in pigs is different between obese and lean animals.
Methods and Results: Group-specific primers were designed to target the 16S rRNA genes of Bacteroidetes and Firmicutes present in the gut. After the validation of their specificity, these primers were used in the real-time PCR quantification of all Bacteria, Firmicutes division, Bacteroidetes division and Bacteroides spp. in the faecal samples of obese and lean pigs from Banna mini-pig inbred line. The obese pigs had a ∼61% fewer percentage (based on all Bacteria) of Bacteroidetes division (P = 0·033) and a ∼56% fewer proportion of Bacteroides spp. (P = 0·047) than the lean pigs. The proportions of both Bacteroidetes and Bacteroides had a negative correlation (P < 0·01) with the body weight.
Conclusion: The results suggested that the fat storage might affect the proportion of Bacteroidetes division in the gut.
Significance and Impact of the Study: The real-time PCR assays developed for Firmicutes and Bacteroidetes will be useful for investigating the composition of gut microbiota.