Auxin production by plant associated bacteria: impact on endogenous IAA content and growth of Triticum aestivum L.
Version of Record online: 9 FEB 2009
© 2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 48, Issue 5, pages 542–547, May 2009
How to Cite
Ali, B., Sabri, A.N., Ljung, K. and Hasnain, S. (2009), Auxin production by plant associated bacteria: impact on endogenous IAA content and growth of Triticum aestivum L. Letters in Applied Microbiology, 48: 542–547. doi: 10.1111/j.1472-765X.2009.02565.x
- Issue online: 9 APR 2009
- Version of Record online: 9 FEB 2009
- 2008/1493: received 30 August 2008, revised 7 December 2008 and accepted 8 December 2008
- axenic conditions;
- endogenous IAA;
- GC–MS analysis;
- indole-3-acetic acid;
- plant associated bacteria
Aims: The aim of this study was to investigate the potential of bacterial strains of Bacillus, Pseudomonas, Escherichia, Micrococcus and Staphylococcus genera associated with wild herbaceous flora to enhance endogenous indole-3-acetic acid (IAA) content and growth of Triticum aestivum var. Inqalab-91.
Methods and Results: Gas chromatography and mass spectrometric (GC–MS) analysis revealed that bacterial strains produced 0·6–8·22 μg IAA ml−1 in the presence of L-tryptophan. Plant microbe experiments showed a significant positive correlation between auxin production by bacterial strains and endogenous IAA content of T. aestivum for GC–MS (r = 0·618; P = 0.05) and colorimetric analysis (r = 0·693; P = 0.01). Similarly, highly significant positive correlation for shoot length (r = 0·627; P = 0.01) and shoot fresh weight (r = 0·626; P = 0.01) was observed with auxin production under axenic conditions. Bacterial inoculations also enhanced shoot length (up to 29·16%), number of tillers (up to 97·35%), spike length (up to 25·20%) and seed weight (up to 13·70%) at final harvest.
Conclusions: Bacterial strains have the ability to increase the endogenous IAA content and growth of T. aestivum var. Inqalab-91.
Significance and Impact of the Study: Microbial strains of wild herbaceous flora can be effectively used to enhance the growth and yield of agronomically important crops.