D. Quistián-Martínez and J. Villatoro-Hernández have contributed equally to this work.
Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis
Version of Record online: 16 JUL 2010
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 51, Issue 4, pages 383–387, October 2010
How to Cite
Quistián-Martínez, D., Villatoro-Hernández, J., Loera-Arias, M.J., Rangel-Colmenero, B.R., Zavala-Flores, L.M., Sepúlveda-Saavedra, J., Guzmán-López, S., Elizondo-Omaña, R.E., Montes-de-Oca-Luna, R. and Saucedo-Cárdenas, O. (2010), Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis. Letters in Applied Microbiology, 51: 383–387. doi: 10.1111/j.1472-765X.2010.02905.x
Present address: J. Villatoro-Hernandez, Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, NED. Kluyver Centre for Genomics of Industrial Fermentation, PO Box 5057, 2600 GA Delft, The Netherlands.
- Issue online: 14 SEP 2010
- Version of Record online: 16 JUL 2010
- 2010/0493: received 26 March 2010, revised 2 July 2010 and accepted 11 July 2010
- antitumour protection;
- Lactococcus lactis;
- modified E7;
Aims: To create and provide a strain of the food-grade bacterium Lactococcus lactis able to efficiently secrete a modified form of the E7 protein from the human papilloma virus (HPV) type-16.
Methods and Results: We cloned the coding sequence of a modified E7 (E7m) from the HPV-16 in a plasmid regulated by the strong expression promoter p59. Secretion of the E7m was made by the signal peptide of the usp45 gene. The E7m was detected by Western blot in the cell-free-medium fraction, showing no degradation or aberrant forms.
Conclusions: We constructed a strain of L. lactis able to secrete efficiently a HPV-16 E7 modified protein with diminished transforming activity.
Significance and Impact of the Study: Human papilloma virus infection is associated with more than 99% of cervical cancers. Immunotherapy targeting E7 to treat HPV-associated cervical malignancies has been demonstrated to be highly efficient. However, native E7 maintains transforming activity. We present this new strain of a food-grade bacterium able to efficiently secrete a HPV-16 E7-modified protein with diminished transforming activity. This new strain could be used as a live vaccine to deliver E7 at a mucosal level and generate antitumour immune responses against HPV-associated tumours.