Isolation of clinically relevant fungal species from solid waste and environment of dental health services
Article first published online: 18 JUL 2010
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 51, Issue 4, pages 370–376, October 2010
How to Cite
Vieira, C.D., De Carvalho, M.A.R., De Resende, M.A., De Menezes Cussiol, N.A., Alvarez-Leite, M.E., Dos Santos, S.G., De Oliveira, M.B., De Magalhães, T.F.F., Silva, M.X., Nicoli, J.R. and De Macêdo Farias, L. (2010), Isolation of clinically relevant fungal species from solid waste and environment of dental health services. Letters in Applied Microbiology, 51: 370–376. doi: 10.1111/j.1472-765X.2010.02907.x
- Issue published online: 14 SEP 2010
- Article first published online: 18 JUL 2010
- 2010/0260: received 11 February 2010, revised 10 April 2010 and accepted 6 July 2010
- airborne fungi;
- dental health services;
- dental solid waste;
- yeast strains
Aims: This study was undertaken to detect, identify and determine antifungal susceptibility of yeast strains isolated from dental solid waste and to evaluate airborne fungi in the Brazilian dental health care environment and in the waste storage room.
Methods and Results: A group of 17 yeast strains were identified by macroscopic and microscopic characteristics, API 20C Aux system and Multiplex PCR. All 104 airborne fungal colonies were identified by macroscopic and microscopic morphology. The CLSI broth microdilution method was utilized as the susceptibility test. Candida parapsilosis was the prevailing yeast species recovered from waste, followed by Rhodotorula glutinis. Three strains of Candida guilliermondii presented minimal inhibitory concentration values considered to be susceptible dose dependent (2 μg ml−1) to voriconazole. Of all airborne fungal species, 69% were recovered from the waste storage room and 31% were recovered from the clinical/surgical environment. Most of them were identified as Cladosporium spp.
Conclusions: These findings reinforce the potential risk of waste handling and point out the need for safe management to minimize the spread of these agents to the environment. Filamentous fungi isolation in almost all sampled environments indicates that a periodic monitoring of airborne microbiota in the dental health care service environment is required.
Significance and Impact of the Study: The survival of yeast strains for 48 h suggests that dental waste should be carefully controlled and monitored.