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Keywords:

  • adenovirus;
  • decontamination;
  • hydrogen peroxide vapour

Abstract

Aims:  Adenovirus contamination can be problematic in various settings including life science laboratories and during pharmaceutical manufacturing processes. Stringent and effective decontamination procedures are necessary to minimize the risk of personnel exposure or product cross-contamination in these settings. Hydrogen peroxide vapour (HPV) is sporicidal, tuberculocidal and fungicidal with proven efficacy against some viruses. We investigate the efficacy of HPV for the inactivation of a recombinant adenovirus.

Methods and Results:  In this study, the survival of a dried recombinant adenovirus (Ad5GFP) was tested before and after HPV exposure to determine the efficacy of HPV at inactivating adenovirus. A > 8-log TCID50 reduction resulted from 45-min exposure to HPV in a microbiological safety cabinet.

Conclusions:  HPV is effective for the inactivation of a recombinant adenovirus.

Significance and impact of the study:  The results suggest that HPV may be useful for adenovirus decontamination in life science laboratories or in manufacturing facilities.