These authors contributed equally to this study and are regarded as joint first authors.
Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40
Article first published online: 18 NOV 2011
© Nanjing Agricultural University. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 54, Issue 1, pages 10–17, January 2012
How to Cite
Zhan, Y., Xu, Q., Yang, M.-M., Yang, H.-T., Liu, H.-X., Wang, Y.-P. and Guo, J.-H. (2012), Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40. Letters in Applied Microbiology, 54: 10–17. doi: 10.1111/j.1472-765X.2011.03165.x
- Issue published online: 8 DEC 2011
- Article first published online: 18 NOV 2011
- Accepted manuscript online: 20 OCT 2011 11:26AM EST
- 2011/1382: received 26 August 2011, revised 11 October 2011 and accepted 12 October 2011
- protective agents;
- rehydration media;
- survival rates;
Aims: The effects of different freeze-drying protective agents on the viabilities of biocontrol strains Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 were investigated.
Method and Results: Several concentrations of protective and rehydration media were tested to improve the survival of biocontrol agents after freeze-drying. The subsequent survival rates during storage and rehydration media of freeze-dried biocontrol strains were also examined.
Conclusions: The results indicated that cellobiose (5%) and d-galactose (5%) gave maximum viability of strains Bu. vietnamiensis B418 and P. agglomerans 2Re40 (98 and 54·3% respectively) while the perfect one (100%) of strain B. cereus AR156 was obtained with sucrose (5%) during freeze-drying, and the highest survival of the three strains was reached when they were rehydrated with 10% nonfat skim milk. In the following storage, the survival rates showed that B. cereus AR156 could still reach 50% after 12 months.
Significance and Impact of the study: This study showed that freeze-drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale-up possibilities and formulation development.