Quantifying dental biofilm growth using cross-polarization optical coherence tomography
Version of Record online: 10 APR 2012
© No claim to US Government works. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology
Letters in Applied Microbiology
Volume 54, Issue 6, pages 537–542, June 2012
How to Cite
Chen, R., Rudney, J., Aparicio, C., Fok, A. and Jones, R.S. (2012), Quantifying dental biofilm growth using cross-polarization optical coherence tomography. Letters in Applied Microbiology, 54: 537–542. doi: 10.1111/j.1472-765X.2012.03243.x
- Issue online: 11 MAY 2012
- Version of Record online: 10 APR 2012
- Accepted manuscript online: 22 MAR 2012 01:43AM EST
- 2012/0039: received 10 January 2012, revised 20 February 2012 and accepted 13 March 2012
- dental biofilms;
- optical coherence tomography;
Aims: Quantifying the ex vivo growth of complex multispecies dental biofilms using cross-polarization 1310-nm optical coherence tomography (CP-OCT) system was investigated.
Methods and Results: Bacterial microcosms, which were derived from plaque samples of paediatric subjects, were incubated in a biofilm reactor system containing discs of different dental materials for 72 h with daily sucrose pulsing (5×). CP-OCT analysis of biofilm mass was validated with crystal violet (CV) assays at various growth stages of these complex biofilms. CP-OCT was able to filter out the back-reflected signals of water layers in the hydrated biofilm and allowed for direct biofilm quantification. The overall depth-resolved scattering intensity of the biofilm showed very strong positive correlation with CV assay quantification (Spearman’s ρ = 0·92) during the growth phase of the biofilm.
Conclusion: CP-OCT was able to quantify the mass of the biofilm by measuring the overall depth-resolved scattering of the biofilm.
Significance and Impact of the Study: CP-OCT has the ability to nondestructively monitor biofilm growth and elucidate the growth characteristics of these microcosms on different dental material compositions.