Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143-0448, USA.
SIRT1 transgenic mice show phenotypes resembling calorie restriction
Article first published online: 13 AUG 2007
Volume 6, Issue 6, pages 759–767, December 2007
How to Cite
Bordone, L., Cohen, D., Robinson, A., Motta, M. C., Van Veen, E., Czopik, A., Steele, A. D., Crowe, H., Marmor, S., Luo, J., Gu, W. and Guarente, L. (2007), SIRT1 transgenic mice show phenotypes resembling calorie restriction. Aging Cell, 6: 759–767. doi: 10.1111/j.1474-9726.2007.00335.x
- Issue published online: 13 AUG 2007
- Article first published online: 13 AUG 2007
- Accepted for publication 14 July 2007
- calorie restriction;
- transgenic mice
We generated mice that overexpress the sirtuin, SIRT1. Transgenic mice have been generated by knocking in SIRT1 cDNA into the β-actin locus. Mice that are hemizygous for this transgene express normal levels of β-actin and higher levels of SIRT1 protein in several tissues. Transgenic mice display some phenotypes similar to mice on a calorie-restricted diet: they are leaner than littermate controls; are more metabolically active; display reductions in blood cholesterol, adipokines, insulin and fasted glucose; and are more glucose tolerant. Furthermore, transgenic mice perform better on a rotarod challenge and also show a delay in reproduction. Our findings suggest that increased expression of SIRT1 in mice elicits beneficial phenotypes that may be relevant to human health and longevity.