Fig. S1 Overexpression of Shc proteins decreases insulin signaling through IRS1/PI3K/Akt pathway. FL83B cells were transfected with indicated plasmids. Serum was overnight deprived from transfected cells and then media were supplemented with indicated concentrations of insulin. Ten minutes later, the cells were washed with ice-cold PBS and lysed. Cell lysates were Western analyzed with indicated antibody. Two independent transfection experiments were performed. Densitometry results of Western blots are presented in the graph, error bars are standard deviations of two experiments.

As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

ACEL_641_sm_FigureS1.tif641KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.