These authors contributed equally to this work.
Genome-wide association study identifies a single major locus contributing to survival into old age; the APOE locus revisited
Article first published online: 5 MAY 2011
© 2011 The Authors. Aging Cell © 2011 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 10, Issue 4, pages 686–698, August 2011
How to Cite
Deelen, J., Beekman, M., Uh, H.-W., Helmer, Q., Kuningas, M., Christiansen, L., Kremer, D., van der Breggen, R., Suchiman, H. E. D., Lakenberg, N., van den Akker, E. B., Passtoors, W. M., Tiemeier, H., van Heemst, D., de Craen, A. J., Rivadeneira, F., de Geus, E. J., Perola, M., van der Ouderaa, F. J., Gunn, D. A., Boomsma, D. I., Uitterlinden, A. G., Christensen, K., van Duijn, C. M., Heijmans, B. T., Houwing-Duistermaat, J. J., Westendorp, R. G. J. and Slagboom, P. E. (2011), Genome-wide association study identifies a single major locus contributing to survival into old age; the APOE locus revisited. Aging Cell, 10: 686–698. doi: 10.1111/j.1474-9726.2011.00705.x
- Issue published online: 12 JUL 2011
- Article first published online: 5 MAY 2011
- Accepted manuscript online: 21 MAR 2011 01:32PM EST
- Accepted for publication 11 March 2011
Fig. S1 Manhattan plot presenting the –log10P from the Cochran-Armitage trend test for the 516,721 SNPs that passed the quality control thresholds in the LLS GWAS.
Fig. S2 Genomic region surrounding rs2075650 (obtained from the UCSC genome browser (http://genome.ucsc.edu/). The physical distances between rs2075650 and rs429358 and between rs2075650 and rs7412 are 16.32 kb and 16.46 kb, respectively.
Fig. S3 Kaplan-Meier curves showing the survival rate over years to follow-up of ApoE 33 carriers with zero (solid line) or one (large dashed line) minor allele(s) of rs2075650 in the LLS, Leiden 85-plus study and Danish 1905 cohort.
Fig. S4 Kaplan-Meier curves showing the survival rate over years to follow-up of carriers of zero (solid line), one (large dashed line), or two (small dashed line) 4 allele(s) of rs429358 in the LLS and Leiden 85-plus study.
Fig. S5 C1 values plotted against the C2 values, both resulting from the multidimensional scaling analysis, of the 403 LLS GWAS cases (green) and the 1670 RS GWAS controls (blue).
Fig. S6 Quantile–quantile plot of expected vs. observed chi-square values for the test statistic from the Cochran-Armitage trend test for 516,721 SNPs that passed the quality control thresholds in the LLS GWAS. The slope of the dashed line represents the genomic inflation factor (λ = 1.027). The shaded region represents the 95% confidence band.
Table S1 SNPs (n = 62) selected for replication analysis, associating at P < 1 × 10−4 with survival into old age in the analysis of the LLS GWAS.
Table S2 (A) Results of the association analysis with survival into old age of the 58 prioritized SNPs from the LLS GWAS in the RS replication study, Leiden 85-plus replication study, Danish replication study, and the meta-analysis. (B) Results of the meta-association analysis with survival into old age of the 58 prioritized SNPs in male cases compared to all controls. (C) Results of the meta-association analysis with survival into old age of the 58 prioritized SNPs in female cases compared to all controls.
Table S3 (A) Results of the prospective analysis of rs2075650 adjusted for rs429358 (4) and rs7412 (2). (B) Results of the prospective analysis of rs429358 (4).
Table S4 Association of LLS GWAS SNPs selected from the AlzGene database [http://www.alzgene.org/, (Bertram et al. 2007)] with survival into old age.
Table S5 Association of LLS GWAS SNPs within a 10 Kb window around FOXO3A and AKT1 with survival into old age.
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