• Open Access

Reversal of age-related oxidative stress prevents hippocampal synaptic plasticity deficits by protecting d-serine-dependent NMDA receptor activation

Authors

  • Coline Haxaire,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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  • Fabrice R Turpin,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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    • Present address: Synaptic plasticity laboratory, Queensland Brain Institute, Saint Lucia, Australia

  • Brigitte Potier,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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  • Myriam Kervern,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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  • Pierre-Marie Sinet,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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  • Gérard Barbanel,

    1. Institut des Biomolecules Max Mousseron, UMR 5247 CNRS-UM1-UM2, Université Montpellier II, Montpellier, 34095, France
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  • Jean-Pierre Mothet,

    1. Equipe ‘gliotransmission et synaptopathies’, Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille, UMR6231-CNRS-Universite Aix-Marseille, Faculté de Médecine secteur Nord 51, Marseille, 13344, France
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  • Patrick Dutar,

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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  • Jean-Marie Billard

    1. Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, Paris, 75014, France
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Jean-Marie Billard, Centre de Psychiatrie et Neurosciences, Université Paris Descartes, Sorbonne Paris Cité, UMR 894, 2 ter rue d’Alésia, Paris, 75014, France. Tel.: + 33 1 40 78 86 47; fax +33 1 45 80 72 93; e-mail: jean-marie.billard@inserm.fr

Summary

Oxidative stress (OS) resulting from an imbalance between antioxidant defenses and the intracellular accumulation of reactive oxygen species (ROS) contributes to age-related memory deficits. While impaired synaptic plasticity in neuronal networks is thought to underlie cognitive deficits during aging, whether this process is targeted by OS and what the mechanisms involved are still remain open questions. In this study, we investigated the age-related effects of the reducing agent N-acetyl-L-cysteine (L-NAC) on the activation of the N-methyl- d-aspartate receptor (NMDA-R) by its co-agonist d-serine, because alterations in this mechanism contribute greatly to synaptic plasticity deficits in aged animals. Long-term dietary supplementation with L-NAC prevented oxidative damage in the hippocampus of aged rats. Electrophysiological recordings in the CA1 of hippocampal slices indicated that NMDA-R-mediated synaptic potentials and theta-burst-induced long-term potentiation (LTP) were depressed in aged animals, deficits that could be reversed by exogenous d-serine. Chronic treatment with L-NAC, but not acute application of the reducing agent, restored potent d-serine-dependent NMDA-R activation and LTP induction in aged rats. In addition, it is also revealed that the age-related decrease in d-serine levels and in the expression of the synthesizing enzyme serine racemase, which underlies the decrease in NMDA-R activation by the amino acid, was rescued by long-term dietary treatment with L-NAC.

 Our results indicate that protecting redox status in aged animals could prevent injury to the cellular mechanisms underlying cognitive aging, in part by maintaining potent NMDA-R activation through the d-serine-dependent pathway.

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