These authors contributed equally to this work.
Aged regulatory T cells protect from autoimmune inflammation despite reduced STAT3 activation and decreased constraint of IL-17 producing T cells
Version of Record online: 22 MAR 2012
© 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 11, Issue 3, pages 509–519, June 2012
How to Cite
Sun, L., Hurez, V. J., Thibodeaux, S. R., Kious, M. J., Liu, A., Lin, P., Murthy, K., Pandeswara, S., Shin, T. and Curiel, T. J. (2012), Aged regulatory T cells protect from autoimmune inflammation despite reduced STAT3 activation and decreased constraint of IL-17 producing T cells. Aging Cell, 11: 509–519. doi: 10.1111/j.1474-9726.2012.00812.x
- Issue online: 11 MAY 2012
- Version of Record online: 22 MAR 2012
- Accepted manuscript online: 28 FEB 2012 01:35PM EST
- Accepted For publication 20 February 2012
Fig. S1 Histological pathology scores of livers (A) and lungs (B) in groups of N = 3 Rag-1−/− mice transferred with 2.5 ´ 105 CD4+ CD25− CD45RBhi T cells from young or aged C57BL/6 mice, or untreated Rag-1−/− mice (Control). Differences between young and aged cell recipients are not significant.
Fig. S2 Histological pathology scores of livers (A) and lungs (B) in Rag-1−/− mice transferred with 5 ´ 105 CD4+ CD25− PD-1+ T cells (PD-1+) or CD4+ CD25− CD45RBhi T cells (CD45RBhi) from aged C57BL/6 mice and sacrificed 56 days later.
Fig. S3 Body weight changes in Rag-1−/− mice transferred with 5 ´ 105 total CD8+ T cells, or CD8+ CD122+ T cells with or without CD4+ CD25hi Tregs (A), or with CD8+ CD122− T cells with or without CD4+ CD25hi Tregs (B) from aged C57BL/6 mice. No difference in peripheral white blood cell counts at 1.5 months (C) and 4.5 months posttransfer (D) of T cells from mice in panels A–B. None of the differences in weight or cell counts was significant between any groups.
Fig. S4. (A) In vitro suppression of aged CD4+ CD25− CD45RBhi T cell (effector) proliferation by aged Tregs or aged CD4+ CD25− PD-1+ T cells. (B) Histological pathology scores of livers and lungs (C) in Rag-1−/− mice transferred with 2.5 ´ 105 young or aged CD4+ CD25− CD45RBhi T cells alone or with 1.25 ´ 105 CD4+ CD25hi age-matched Tregs.
Fig. S5 Histological pathology scores of livers (A) and lungs (B) in Rag-1−/− mice transferred with 1 ´ 106 CD4+ CD25− CD45RBhi T cells alone or with 5 ´ 105 CD4+ CD25− PD-1+ T cells from aged mice.
Fig. S6 Analysis of pSTAT3 in aged and young immune cells. (A) CD4+ Foxp3-RFP− (naïve) T cells. (B) Young and (C) aged CD4+ Foxp3-RFP+ (Tregs), CD4+ Foxp3-RFP− (naïve), CD8+ T cells, and B cells by flow cytometry after stimulation of the cells with indicated concentrations of IL-6.
Fig. S7 Histological pathology scores of livers (A) and lungs (B) in young and aged Foxp3DTR mice were treated with PBS or DT for 7 days and sacrificed the day after.
Fig. S8 CD62L and CD45RB expression in CD4+ CD25− PD-1+(left) or CD4+ CD25− PD-1−(right) T cells. Representative flow cytometry panel of 3 experiments with similar results using n = 10–12 mice/group is shown.
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