SEARCH

SEARCH BY CITATION

Fig. S1 Histological pathology scores of livers (A) and lungs (B) in groups of N = 3 Rag-1−/− mice transferred with 2.5 ´ 105 CD4CD25− CD45RBhi T cells from young or aged C57BL/6 mice, or untreated Rag-1−/− mice (Control). Differences between young and aged cell recipients are not significant.

Fig. S2 Histological pathology scores of livers (A) and lungs (B) in Rag-1−/− mice transferred with 5 ´ 105 CD4CD25− PD-1+ T cells (PD-1+) or CD4CD25− CD45RBhi T cells (CD45RBhi) from aged C57BL/6 mice and sacrificed 56 days later.

Fig. S3 Body weight changes in Rag-1−/− mice transferred with 5 ´ 105 total CD8+ T cells, or CD8CD122+ T cells with or without CD4CD25hi Tregs (A), or with CD8CD122 T cells with or without CD4CD25hi Tregs (B) from aged C57BL/6 mice. No difference in peripheral white blood cell counts at 1.5 months (C) and 4.5 months posttransfer (D) of T cells from mice in panels A–B. None of the differences in weight or cell counts was significant between any groups.

Fig. S4. (A) In vitro suppression of aged CD4CD25− CD45RBhi T cell (effector) proliferation by aged Tregs or aged CD4CD25− PD-1+ T cells. (B) Histological pathology scores of livers and lungs (C) in Rag-1−/− mice transferred with 2.5 ´ 105 young or aged CD4CD25− CD45RBhi T cells alone or with 1.25 ´ 105 CD4CD25hi age-matched Tregs.

Fig. S5 Histological pathology scores of livers (A) and lungs (B) in Rag-1−/− mice transferred with 1 ´ 106 CD4CD25− CD45RBhi T cells alone or with 5 ´ 105 CD4CD25− PD-1+ T cells from aged mice.

Fig. S6 Analysis of pSTAT3 in aged and young immune cells. (A) CD4Foxp3-RFP (naïve) T cells. (B) Young and (C) aged CD4Foxp3-RFP+ (Tregs), CD4Foxp3-RFP (naïve), CD8+ T cells, and B cells by flow cytometry after stimulation of the cells with indicated concentrations of IL-6.

Fig. S7 Histological pathology scores of livers (A) and lungs (B) in young and aged Foxp3DTR mice were treated with PBS or DT for 7 days and sacrificed the day after.

Fig. S8 CD62L and CD45RB expression in CD4CD25− PD-1+(left) or CD4CD25− PD-1(right) T cells. Representative flow cytometry panel of 3 experiments with similar results using n = 10–12 mice/group is shown.

As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

FilenameFormatSizeDescription
ACEL_812_sm_FigS1.jpg25KSupporting info item
ACEL_812_sm_FigS2.jpg31KSupporting info item
ACEL_812_sm_FigS3.jpg431KSupporting info item
ACEL_812_sm_FigS4.jpg79KSupporting info item
ACEL_812_sm_FigS5.jpg24KSupporting info item
ACEL_812_sm_FigS6.jpg83KSupporting info item
ACEL_812_sm_FigS7.jpg35KSupporting info item
ACEL_812_sm_FigS8.jpg80KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.