Genome-wide miRNA signatures of human longevity

  1. Abdou ElSharawy1,†,
  2. Andreas Keller2,3,†,
  3. Friederike Flachsbart1,
  4. Anke Wendschlag4,
  5. Gunnar Jacobs5,
  6. Nathalie Kefer4,
  7. Thomas Brefort4,
  8. Petra Leidinger3,
  9. Christina Backes3,
  10. Eckart Meese3,
  11. Stefan Schreiber1,6,
  12. Philip Rosenstiel1,
  13. Andre Franke1,‡,
  14. Almut Nebel1,‡

Article first published online: 30 MAY 2012

DOI: 10.1111/j.1474-9726.2012.00824.x

Issue

Aging Cell

Aging Cell

Volume 11, Issue 4, pages 607–616, August 2012

Additional Information

How to Cite

ElSharawy, A., Keller, A., Flachsbart, F., Wendschlag, A., Jacobs, G., Kefer, N., Brefort, T., Leidinger, P., Backes, C., Meese, E., Schreiber, S., Rosenstiel, P., Franke, A. and Nebel, A. (2012), Genome-wide miRNA signatures of human longevity. Aging Cell, 11: 607–616. doi: 10.1111/j.1474-9726.2012.00824.x

Author Information

  1. 1

    Institute of Clinical Molecular Biology, Christian-Albrechts-University, Schittenhelmstraße 12, D-24105 Kiel, Germany

  2. 2

    Biomarker Discovery Center, Im Neuenheimer Feld 519, D-69120 Heidelberg, Germany

  3. 3

    Department of Human Genetics, Saarland University, Medical Department, D-66421 Homburg, Germany

  4. 4

    Febit biomed gmbh, Im Neuenheimer Feld 519, D-69120 Heidelberg, Germany

  5. 5

    Biobank Popgen, Institute of Experimental Medicine, Christian-Albrechts-University, Niemannsweg 11, D-24105 Kiel, Germany

  6. 6

    Clinic for Internal Medicine I, University Hospital of Schleswig-Holstein, Schittenhelmstraße 12, D-24105 Kiel, Germany

  1. †

    These authors contributed equally to this work.

  2. ‡

    These authors shared senior authorship.

*Almut Nebel, PhD, Institute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, Schittenhelmstraße 12, 24105 Kiel, Germany. Tel.: +49 431 597 1373; fax: +49 431 597 2196; e-mail: a.nebel@mucosa.de

  1. †

    These authors contributed equally to this work.

  2. ‡

    These authors shared senior authorship.

Publication History

  1. Issue published online: 16 JUL 2012
  2. Article first published online: 30 MAY 2012
  3. Accepted manuscript online: 26 APR 2012 02:43AM EST
  4. Accepted for publication 7 April 2012

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Fig. S1 Aging-related miRNA changes in expression (median values from 15 LLI and 55 younger individuals, respectively).

Fig. S2 Area under the receiver operator characteristic curve (AUC) of the three differently regulated aging-related biomarkers miR-30c, miR-126 and miR-106a.

Fig. S3 (a–h) qRT-PCR amplification plots (CT values) for miR-106a (3a), miR-126 (3b), miR-30d (3c), miR-320d (3d), miR-320b (3e), miR-20a (3f), miR-144* (3g), and miR-18a (3h) in LLI versus younger control samples.

Fig. S4 A network of reported connections between significantly differentially regulated aging-related miRNAs and diseases.

Fig. S5 Genomic distribution of differentially regulated aging-related miRNAs.

Fig. S6 Genomic density distribution of the differentially regulated aging-related miRNAs.

Fig. S7 Ages (mean and standard deviation) of the investiagted individuals in the different experimental stages.

Table S1 Statistical results of all miRNAs investigated in the screening stage using microarrays.

Table S2 Results of the replication of miR-106a, miR-126 and miR-30d using qRT-PCR compared to the validation and microarray-expression results.

Table S3 Target genes of miRNAs down-regulated in long-lived individuals.

Table S4 Key numbers for the reported relations of age-associated miRNAs to different diseases.

Table S5 Disease enrichment of differentially regulated aging-related miRNAs.

Table S6 Relation of age-associated miRNAs to disease.

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