Zhonghao Yu, Guangju Zhai and Paula Singmann contributed equally.
Human serum metabolic profiles are age dependent
Version of Record online: 27 AUG 2012
© 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 11, Issue 6, pages 960–967, December 2012
How to Cite
Yu, Z., Zhai, G., Singmann, P., He, Y., Xu, T., Prehn, C., Römisch-Margl, W., Lattka, E., Gieger, C., Soranzo, N., Heinrich, J., Standl, M., Thiering, E., Mittelstraß, K., Wichmann, H.-E., Peters, A., Suhre, K., Li, Y., Adamski, J., Spector, T. D., Illig, T. and Wang-Sattler, R. (2012), Human serum metabolic profiles are age dependent. Aging Cell, 11: 960–967. doi: 10.1111/j.1474-9726.2012.00865.x
Carried out at the Research Unit of Molecular Epidemiology, Helmholtz ZentrumMünchen, 85764 Neuherberg, Germany, and the Department of Twin Research and Genetic Epidemiology, King’s College London, London, UK.
Co-corresponding authors. Tim D. Spector, Department of Twin Research and Genetic Epidemiology, King’s College London, London, UK and Thomas Illig, Hannover Unified Biobank, Hannover Medical School, 30625 Hannover, Germany. Tel.: +49 89 3187 3978; fax: +49 89 3187 2428; e-mails: email@example.com; firstname.lastname@example.org
Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms
- Issue online: 15 NOV 2012
- Version of Record online: 27 AUG 2012
- Accepted manuscript online: 26 JUL 2012 12:19PM EST
- Accepted for publication 16 July 2012
Fig. S1 Correlation matrix for women in KORA F4. Values of Pearson correlation coefficients between every pair of metabolites were displayed in a heat map. Red indicates a positive correlation, while yellow corresponds to a negative-to-zero correlation. Grey boxes represent groups of metabolites clustered together. AA, amino acid; AC, acylcarnitines; PC aa, phosphatidylcholinediacyl; PC ae, phosphatidylcholine acyl-alkyl; and lyso PC a, lysophosphatidylcholine acyl.
Fig. S2 Heat map of the fold standard deviation changes between ages, and clustering of these changes over all ages, in 1124 males from KORA F4. The heat map shows changes of x-fold standard deviation from the overall mean concentration for each age year in a color-coded depiction. Green squares represent a decrease, and red squares an increase. Grey boxes represent groups of metabolites with similar changes, with number of metabolites in parentheses. Metabolite names in red indicate our set of 12 metabolites. AA, amino acid; AC, acylcarnitines; PC aa, phosphatidylcholinediacyl; PC ae, phosphatidylcholine acyl-alkyl; and lyso PC a, lysophosphatidylcholine acyl.
Fig. S3 Concentration trends of 13 potentialbiomarkers in women from KORA F4. Age is shown on thex-axis; metabolite concentration (μM) plotted, on they-axis; grey area, 95% prediction interval. Plots weretruncated from 1–99 percentiles of observations.
Fig. S4 Concentration trends of 12 selectedmetabolites in males from KORA F4. Age is shown on thex-axis; metabolite concentration (μM) plotted, on they-axis; grey area, 95% prediction interval. Plots weretruncated from 1–99 percentiles of observations.
Fig. S5 Concentration trends of the set of 13metabolites in women from the TwinsUK study. Age is shown on thex-axis; metabolite concentration (μM) plotted, on they-axis; grey area, 95% prediction interval. Plots weretruncated from 1–99 percentiles of observations. Note thatcurve progression at the margins should not be overrated, as n islow.
Table S1 Characteristics of 163 metabolites inKORA F4. Abbreviations and full biochemical names of 163metabolites are shown in the first and second columns,respectively. The next three columns display correlationcoefficients (r) between two duplicated measurements on 144re-measured samples, the percentage of 3080 individuals above thelimit of detection (LOD), and the mean value of the coefficient ofvariance (CV) of the three quality controls, respectively. The nextcolumn indicates the status (used/excluded) for each metabolite.The mean and standard deviation of the concentration of each usedmetabolites, along with their correlation with BMI, are listed inthe last two columns.
Table S2 Potential biomarkers for age in menfrom KORA F4. Mean concentrations and standard deviation, alongwith the beta and P values from the regression models, are displayed in the table for the 12 potential age biomarkers in males from KORA F4.
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