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Reliability of different blood indices to explore the oxidative stress in response to maximal cycling and static exercises

Authors

  • Jean Guillaume Steinberg,

    1. Service des Explorations Fonctionnelles Respiratoires, Hôpital Nord, Assistance Publique-Hôpitaux de Marseille and Laboratoire de Physiopathologie Respiratoire (UPRES EA 2201), Faculté de Médecine, Institut Jean Roche, Université de la Méditerranée, Marseille, France
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  • Stéphane Delliaux,

    1. Service des Explorations Fonctionnelles Respiratoires, Hôpital Nord, Assistance Publique-Hôpitaux de Marseille and Laboratoire de Physiopathologie Respiratoire (UPRES EA 2201), Faculté de Médecine, Institut Jean Roche, Université de la Méditerranée, Marseille, France
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  • Yves Jammes

    1. Service des Explorations Fonctionnelles Respiratoires, Hôpital Nord, Assistance Publique-Hôpitaux de Marseille and Laboratoire de Physiopathologie Respiratoire (UPRES EA 2201), Faculté de Médecine, Institut Jean Roche, Université de la Méditerranée, Marseille, France
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Pr. Yves Jammes, Laboratoire de Physiopathologie Respiratoire (UPRES EA 2201), Faculté de Médecine, Institut Jean Roche, Boulevard Pierre Dramard, 13916 cedex 20 Marseille, France
E-mail: jammes.y@jean-roche.univ-mrs.fr

Summary

This study compares the changes in four blood markers of exercise-induced oxidative stress in response to exercise protocols commonly used to explore the global muscle performance at work (maximal incremental cycle) and endurance to fatigue of selected muscles (static handgrip and thumb adduction). Cycling and static exercises allow the muscle to work in aerobic and anaerobic conditions, respectively. Healthy adults performed an incremental cycling exercise until volitional exhaustion and, on separated days, executed infra-maximal static thumb adduction and handgrip until exhaustion. Exercise-induced oxidative stress was assessed by the increased plasma concentration of thiobarbituric acid reactive substances (TBARS), the consumption of plasma reduced ascorbic acid (RAA), and erythrocyte reduced glutathione (GSH) antioxidants, and the changes in the total antioxidant status (TAS) of plasma. Five minutes after the end of the incremental cycling exercise, we measured a peak increase in TBARS level, maximal consumption of GSH and RAA, and a modest but significant decrease in TAS concentration. In response to both static thumb adduction and handgrip, significant variations of TBARS, GSH and RAA occurred but we did not measure any significant change in TAS level throughout the 20-min recovery period of both exercise bouts. The present study shows that only the changes in TBARS, GSH and RAA explore both dynamic and static exercises. In addition, TAS measurement does not seem to represent a reliable and unique tool to explore exercise-induced oxidative stress, at least during isometric efforts that allow the muscle to work under anaerobic condition.

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