The study aims to use lime mortars and plasters to radiocarbon date Nabatean and Islamic structures from Petra and Udruh, south Jordan. Fifteen samples from seven structures were characterized by thin-section, scanning electron and cathodoluminescence microscopy. The lime binders of all the samples and the organic inclusions from nine samples were AMS radiocarbon dated. The dates and the historical data of the samples were compared with each other. The results showed an agreement between the radiocarbon dates of the lime binders and the organic inclusions and the historical data for most of the samples. The radiocarbon dates of the lime binders supported by the radiocarbon dates of the organic inclusions and the archaeological data were helpful in reconstructing a rather precise chronology of the studied structures.