This study explores the potential of carbon and oxygen isotope data from human enamel carbonate (n = 50) to contribute to mobility studies in the Caribbean. Most oxygen and carbon isotope results display limited variation, with substantial overlap between islands. However, a few individuals from El Chorro de Maíta, Cuba possess relatively low δ18O and high δ13C. Two of these individuals were previously identified as possible non-Antillean immigrants on the basis of strontium isotopes, and bioarchaeological and mortuary evidence. The carbon and oxygen isotope results provide additional evidence supporting the proposed non-local origins of these individuals and contribute to explorations of their potential natal origins.