Hemolysis produced by peristaltic pump infusion of canine whole blood was studied in fresh (3-hours-old) and stored (21-day-old) blood units. Blood was pumped at infusion rates of 50, 100, and 200 ml/hr and compared to gravity flow blood at the same rates. IVAC 530 and 560 (peristaltic) and the Animed Flowset 100 (rotaty) pumps were evaluated.
In the fresh blood group, the IVAC 530 did not produce significant hemolysis at any rate compared togravity infusion. Blood pumped through the IVAC 560 and the Animed Flowset 100 was significantly hemolyzed when compared to blood administered by gravity flow.
Free plasma hemoglobin was higher in stored blood than in fresh blood for all groups. The Animed Flowset 100 demonstrated significant hemolysis at 50 and 100 ml/hr flow rates compared to free-dripped blood, the IVAC 530, and the IVAC 560. All pumps hemolyzed blood more at 100 and 200 ml/hr than at 50 ml/hr.
When fresh blood is transfused, it can be pumped through an IVAC 530 at 50, 100, or 200 ml/hr without significant hemolysis. The IVAC 560 produces an intermediate level of hemolysis. The Animed Flowset 100 is not recommended for pumping whole blood. Blood that has been stored for 21 days appears to have a much higher level of hemolysis than fresh blood at all infusion rates.