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Thromboelastography in healthy horses and horses with inflammatory gastrointestinal disorders and suspected coagulopathies

Authors

  • Jose L. Mendez-Angulo DVM,

    1. Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, The Ohio State University, Columbus, OH
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  • Margaret C. Mudge VMD, DACVS, DACVECC,

    1. Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, The Ohio State University, Columbus, OH
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  • Paulo Vilar-Saavedra DVM, MS,

    1. Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, The Ohio State University, Columbus, OH
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  • Nicole Stingle,

    1. Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, The Ohio State University, Columbus, OH
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  • C. Guillermo Couto DVM, DACVIM

    1. Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, The Ohio State University, Columbus, OH
    2. OSU Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210.
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  • Supported in part by Fundacion Caja Madrid International Fellowship Program (Dr. Mendez-Angulo), Madrid, Spain.

  • Presented in part at the 15th International Veterinary Emergency and Critical Care Symposium, Chicago, IL, September 9–13, 2009.

  • The authors declare no conflict of interest.

Address correspondence and reprint requests to
Dr. J.L. Mendez-Angulo, College of Veterinary Medicine, Veterinary Medical Center, University of Minnesota, 1365 Gortner Ave, St. Paul, MN 55108, USA.
Email: v12meanj@uco.es
Submitted October 31, 2009; Accepted August 11, 2010.

Abstract

Objectives – To evaluate the use of citrated recalcified (nonactivated) thromboelastography (TEG) in healthy horses and horses with colitis and suspected coagulopathies.

Design – Prospective, observational study conducted between October 2007 and June 2009.

Setting – Veterinary Teaching Hospital.

Animals – Forty-five healthy adult horses and 12 sick adult horses with colitis and prolonged prothrombin time (PT) or activated partial thromboplastin time (aPTT).

Interventions – None.

Measurements and Main Results – Whole blood was collected on admission. Coagulation profile (PT, aPTT, platelet count, and fibrinogen concentration) and citrated recalcified whole blood TEG analysis (R-time [R], K-time [K], angle [α], maximum amplitude [MA], G value [G], lysis at 60 min [LY60]) were evaluated. Mean values (SD) for TEG parameters in healthy horses were: R=10.4 (3.1) minutes; K=3.5 (1.2) minutes; α=46.3 (11.0)°; MA=55.6 (5.1) mm; G=6,429 (1,341) dyn/cm2, and LY60=5.1 (2.4)%. Mean coefficients of variation for intra-assay/interindividual variability in healthy horses were: R=4.7%/30.7%, K=4.8%/35.3%, α=4.4%/23.8%, MA=1.4%/9.3%, G=3.4%/20.8%, and LY60=13.1%/47.7%, respectively. Horses with colitis and prolonged PT and/or aPTT had longer mean values for R (P<0.001) and K (P<0.001), narrower mean α (P<0.001), decreased mean MA (P=0.001), and smaller mean G (P=0.02); changes consistent with hypocoagulability.

Conclusions – Citrated recalcified (nonactivated) TEG demonstrated changes consistent with hypocoagulability in horses with colitis that had preidentified coagulation abnormalities. This technique has high interindividual variability and low intra-assay variability. TEG may be useful for detecting hypocoagulable states in horses with colitis and suspected coagulopathies.

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