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Keywords:

  • bleeding disorders;
  • immune-mediated thrombocytopenia;
  • platelet storage;
  • primary hemostasis;
  • transfusions

Abstract

Objective

To examine the safety and feasibility of using lyophilized platelets (LYO) and fresh platelet concentrate (FRESH) in bleeding thrombocytopenic dogs.

Design

Preliminary prospective randomized clinical trial.

Setting

Two private referral centers and 3 university teaching hospitals.

Animals

Thirty-seven dogs with a complaint of hemorrhage associated with thrombocytopenia (platelet count <70 × 109/L [70,000/μL], a hematocrit >15%, and that had received neither vincristine nor platelet-containing transfusions within 72 h of enrollment were studied.

Interventions

Animals were randomized to receive LYOor FRESH, dosed according to weight. Physical examination, complete blood counts, and coagulation testing (prothrombin time and activated partial thromboplastin time) were performed at enrollment. Physical examinations were also performed immediately post transfusion, and at 1 and 24 h after transfusion. Complete blood counts were repeated immediately post transfusion and at 24 h. Collected data included bleeding score (BLS), response to transfusion, adverse reactions, hospitalization time, need for additional transfusions, survival to discharge, and 28-d survival.

Measurements and Main Results

Twenty-two dogs received LYOand 15 received FRESH. There was no difference between groups in age, weight, BLS, platelet count, white blood cell count, hematocrit, or presence of melena. There was no difference between groups in transfusion reaction rates, the need for additional transfusions, 24-h BLS, hospitalization time, survival to discharge, or 28-d survival.

Conclusions

Transfusion of LYO was feasible and associated with a low transfusion reaction rate in this limited study of thrombocytopenic canine patients presenting with mild-to-severe hemorrhage. LYOwere easy to use and provided storage advantages over FRESH. Further study of this product, including examination of efficacy and platelet life span, is warranted.