Get access

Assessment of acute moderate hyperglycemia on traditional and thromboelastometry coagulation parameters in healthy adult horses

Authors


  • Funding for this study was provided by the University of Illinois.

  • Dr. Wilkins is an assistant editor for the journal but only participated on the peer review process as an author. The authors declare no other conflict of interests.

  • Presented in part at the 16th IVECCS, San Antonio, Texas, September 2010.

Address correspondence and reprint requests to K. Lascola, Department of Veterinary Clinical Medicine, University of Illinois, 1008 W Hazelwood Drive, Urbana, IL 61802.

Email: k.lascola@illinois.edu

Abstract

Objective

To determine whether experimentally induced acute moderate hyperglycemia is associated with coagulation activation in healthy adult horses.

Design

Prospective experimental study.

Setting

University veterinary teaching hospital.

Animals

Six healthy adult horses.

Interventions

Hyperglycemia (10.0–13.3 mmol/L [180–240 mg/dL]) was induced and maintained for 6 hours using a hyperglycemic clamp technique. Blood glucose concentrations were assessed using a point of care (POC) glucometer at 10- and 20-minute intervals throughout the hyperglycemic clamp procedure. Platelet count, fibrinogen concentration, prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin antithrombin complex level (TAT), and thromboelastometry (TEM) were determined before and after jugular catheter placement, prior to glucose administration, and at 3 and 6 hours of sustained hyperglycemia. Data were analyzed by repeated measures analysis of variance with significance defined as P < 0.05.

Measurements and Main Results

All horses maintained blood glucose concentration >10.0 mmol/L (>180 mg/dL) throughout the duration of the hyperglycemic clamp with a mean concentration of 11.9 ± 0.3 mmol/L [216 ± 6 mg/dL] as measured by the POC glucometer. No significant difference was found for any evaluated parameter associated with sustained hyperglycemia. Intravenous catheter placement resulted in a significant increase in mean TAT (0.8 ± 0.3 μg/L pre-catheter, 2.3 ± 0.8 μg/L post-catheter; P = 0.008).

Conclusions

Acute, moderate hyperglycemia in healthy adult horses does not have a detectable effect on coagulation based on evaluated parameters. Jugular catheter placement results in a transient increase in thrombin generation as determined by increased TAT concentrations.

Ancillary