Department of Pharmacology, University College Hospital, Ibadan. Nigeria
Changes in sodium pool and kinetics of sodium transport in frog skin produced by amiloride
Article first published online: 19 JUL 2012
1970 British Pharmacological Society
British Journal of Pharmacology
Volume 39, Issue 1, pages 99–109, May 1970
How to Cite
SALAKO, L. A. and SMITH, A. J. (1970), Changes in sodium pool and kinetics of sodium transport in frog skin produced by amiloride. British Journal of Pharmacology, 39: 99–109. doi: 10.1111/j.1476-5381.1970.tb09559.x
- Issue published online: 19 JUL 2012
- Article first published online: 19 JUL 2012
- Received November 6, 1969
- 1. Amiloride produces a decrease in size of the active sodium transport pool of isolated frog skin.
- 2. Rate coefficients for sodium movement into and out of the transporting cells across the outside membrane are decreased by amiloride. The rate coefficient for sodium extrusion across the inside membrane is not significantly affected.
- 3. In the presence and in the absence of amiloride, the relation of sodium transport to outside sodium concentration exhibits similar saturation kinetics but amiloride reduces sodium transport rate at every sodium concentration of the outside solution.
- 4. Labelling of skin with 14C-amiloride from the outside solution is significantly greater than labelling with 14C-inulin.
- 5. The results of these studies suggest that amiloride reacts with sites on the outside membrane of the transporting cells as a result of which the rate of sodium movement across this membrane is diminished.