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  • 1
    Atropine (10−5m) enhanced the release of [3H]-acetylcholine from rat isolated hemidiaphragms, previously incubated with [3H-methyl]-choline, stimulated via their phrenic nerves.
  • 2
    Oxotremorine (10−5m) did not affect the stimulated release of [3H]-acetylcholine but antagonized the facilitatory effects of atropine (10−5m).
  • 3
    It is suggested that there are presynaptic inhibitory muscarinic receptors that modulate the release of acetylcholine in the phrenic nerves of the rat.