British Journal of Pharmacology
Explore this journal >

The regulation of δ-opiate receptor density on 108CC15 neuroblastoma × glioma hybrid cells

Authors

Abstract

  • 1The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma × glioma hybrid cells has been studied.
  • 2Cell differentiation by culture in the presence of N6-O2-dibutyryl adenosine 3′,5′-cyclic monophosphate induced a three fold increase in opiate receptor density.
  • 3When the cells were grown in the presence of 10−5 m morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls.
  • 4Culture in the presence of 10−7md-Ala2-d-Leu5-enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment.
  • 5The down regulation process could be inhibited by continued exposure to d-Ala2 d-Leu5-enkephalin at concentrations greater than 4 nm; below this concentration down regulation was rapid and irreversible.
  • 6A model to explain these observations is described.

Ancillary

Article Information

DOI

10.1111/j.1476-5381.1984.tb10757.x

Format Available

Full text: PDF

1984 British Pharmacological Society

Request Permissions

Publication History

  • Issue online:
  • Version of record online:
  • Received July 6, 1983

References

  • AMSTERDAM, A., BERKOWITZ, A., NIMROD, A. & KOHEN, F. (1980). Aggregation of luteinising hormone receptors in granulosa cells: a possible mechanism of desensitisation to the hormone. Proc. natn. Acad. Sci. U.S.A., 77, 3440–3444.
  • BLANCHARD, S.G., CHANG, K. J. & CUATRECASAS, P. (1983). Characterisation of the association of tritiated enkephalin with neuroblastoma cells under conditions optimal for receptor down regulation. J. biol. Chem., 28, 1092–1097.
  • BRANDT, M., FISCHER, K., MORODER, L., WÜNSCH, E. & HAMPRECHT, B. (1976). Enkephalin evokes biochemical correlates of opiate tolerance and dependence in neuroblastoma × glioma hybrid cells. FEBS Letters, 68, 38–40.
  • BURT, R. & SNYDER, S.H. (1975). Thyrotropin releasing hormone (TRH): apparent receptor binding in rat brain membranes. Brain Res., 93, 309–328.
  • CARPENTER, G. & COHEN, S. (1976). 125I-labelled human epidermal growth factor: binding, internalization and degradation in human fibroblast. J. cell. biol., 71, 159–171.
  • CHANG, K.J. & CUATRECASAS, P. (1979). Multiple opiate receptors: enkephalin and morphine bound to receptors of different specificity. J. biol. Chem., 254, 2610–2618.
  • CHANG, K.J., MILLER, R.J. & CUATRECASAS, P. (1978). Interaction of enkephalin with opiate receptors in intact cultured cells. Molec. Pharmac., 14, 951–970.
  • CLENDENINN, N.J., PETRAITIS, M. & SIMON, E.J. (1976). Ontological development of opiate receptors in rodent brain. Brain Res., 118, 157–160.
  • HAIGLER, H.T., ASH, J.F., SINGER, S.J. & COHEN, S. (1978). Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431. Proc. natn. Acad. Sci. U.S.A. 75, 3317–3321.
  • HAMPRECHT, B. (1974). Cell cultures as model system for studying the biochemistry of differentiated function of nerve cells. In 25 Kolloquium der Gesellschaft fur Biologische Chemie. ed. Jaenicke, L. pp. 391–423.
  • HAMPRECHT, B. (1977). Structural, electrophysiological, biochemical and pharmacological properties of neuroblastoma × glioma hybrids in cell culture. Int. Rev. Cytol., 49, 99–170.
  • HAZUM, E., CHANG, K. J. & CUATRECASAS, P. (1979). Opiate receptors of neuroblastoma cells: occurrence in clusters on the cell surface. Science, 206, 1077–1079.
  • HAZUM, E., CUATRECASAS, P., MARIAN, J. & CONN, P.M. (1980). Receptor mediated internalization of fluorescent gonadotropin-releasing hormone by pituitary gonadotrophs. Proc. natn. Acad. Sci. U.S.A., 77, 6692–6695.
  • JOHNSTON, E.M., ANDREWS, R.Y. & BRADSHAW, A.A. (1978). Characterization of the retrograde transport of nerve growth factor using high specific activity (125I]-NGF. Brain Res., 150, 319–331.
  • KLEE, W.A. & NIRENBERG, M. (1974). A neuroblastoma × glioma hybrid cell line with morphine receptors. Proc. natn. Acad. Sci. U.S.A., 71, 3474–3477.
  • LAMPERT, A., NIRENBERG, M. & KLEE, W.A. (1976). Tolerance and dependence evoked by an endogenous opiate peptide. Proc. natn. Acad. Sci. U.S.A., 73, 3165–3167.
  • LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L. & RANDALL, P.J. (1951). Protein measurement with the folin-phenol reagent. J. biol. Chem., 193, 265–275.
  • MUKHERJEE, C., CARON, M.G. & LEFKOWITZ, R.J. (1975). Catecholamine induced subsensitivity of adenylate cyclase associated with loss of β-adrenergic receptor binding sites. Proc. natn. Acad. Sci. U.S.A., 72, 1945–1949.
  • SCATCHARD, G. (1949). The attraction of proteins for small molecules and cells. Ann. N.Y. Acad. Sci., 51, 660–672.
  • SCHLESSINGER, J., SCHECHTER, Y., WILLINGHAM, M.C. & PASTAN, I. (1978). Direct visualisation of binding, aggregation and internalization of insulin and epidermal growth factor on human fibroblast cells. Proc. natn. Acad. Sci. U.S.A., 75, 2659–2663.
  • SCHLESSINGER, J., VANOBBERGHEN, E. & KAHN, C.R. (1980). Insulin and antibodies against insulin receptor cap on the membrane of cultured human lymphocytes. Nature, 286, 729–731.
  • SHARMA, S.K., KLEE, W.A. & NIRENBERG, M. (1975a). Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance. Proc. natn. Acad. Sci. U.S.A., 72, 3092–3096.
  • SHARMA, S.K., NIRENBERG, M. & KLEE, W.A. (1975b). Morphine receptors as regulators of adenylate cyclase activity. Proc. natn. Acad. Sci. U.S.A., 72, 590–594.
  • SU, Y. F., HARDEN, T.K. & PERKINS, J.P. (1979). Isoproterenol induced desensitization of adenylate cylcase in human astrocytoma cells. J. biol. Chem., 254, 38–49.
  • SU, Y. F., HARDEN, T.K. & PERKINS, J.P. (1980). Catecholamine specific desensitization of adenylate cyclase. J. biol. Chem., 255, 7410–7419.
  • TRABER, J., FISCHER, K., LATZIN, S. & HAMPRECHT, B. (1975). Morphine antagonises action of prostoglandin in neuroblastoma and neuroblastoma × glioma hybrid cells. Nature, 253, 120–122.
  • WAHLSTROM, A., BRANDT, M., MORODER, L., WUNSCH, E., LINDEBERG, G., RAGNARSSON, U. TERENIUS, L. & HAMPRECHT, B. (1977). Peptides related to beta-lipotropin with opiate activity effects on levels of adenosine 3′,5′ cyclic monophosphate in neuroblastoma × glioma hybrid cells. FEBS Lett., 77, 28–32.
  • WESSELS, M.R., MULLIKIN, D. & LEFTOWITZ, R.J. (1978). Differences between agonist binding following adrenergic receptor desensitization. J. biol. Chem., 253, 3371–3373.

Related content

Articles related to the one you are viewing

Citing Literature

  • Number of times cited: 4
  1. 1Ana Vicente-Sanchez, Laura Segura, Amynah A. Pradhan, The delta opioid receptor tool box, Neuroscience, 2016, 338, 145CrossRef
  2. 2P Schmidt, C Schmolke, F Mußhoff, M Menzen, C Prohaska, B Madea, Numerical density of δ-opioid receptor expressing neurons in the frontal cortex of drug-related fatalities, Forensic Science International, 2000, 113, 1-3, 423CrossRef
  3. 3Joan P. Schwartz, Chronic exposure to opiate agonists increases proenkephalin biosynthesis in NG108 cells, Molecular Brain Research, 1988, 3, 2, 141CrossRef
  4. 4C.R. Shell, M.A. Moses, D.G. Hughes, Tolerance and withdrawal are associated with ligand dependent receptor regulation in vitro and in vivo, Neuropeptides, 1984, 5, 1-3, 23CrossRef