Frequency-dependent inhibition of neuronal activity by lappaconitine in normal and epileptic hippocampal slices
Article first published online: 19 JUL 2012
1996 British Pharmacological Society
British Journal of Pharmacology
Volume 118, Issue 3, pages 577–584, June 1996
How to Cite
Ameri, A., Metzmeier, P. and Peters, T. (1996), Frequency-dependent inhibition of neuronal activity by lappaconitine in normal and epileptic hippocampal slices. British Journal of Pharmacology, 118: 577–584. doi: 10.1111/j.1476-5381.1996.tb15440.x
- Issue published online: 19 JUL 2012
- Article first published online: 19 JUL 2012
- Received November 13, 1995; Revised January 11, 1996; Accepted February 13, 1996
- extracellular recordings;
- 1Extracellular recording of the stimulus-evoked population spike in the CA1 region of rat hippocampal slices in vitro was performed in order to investigate whether lappaconitine affects neuronal excitability. Lappaconitine is a diterpene alkaloid of plants of the Aconitum genus and has analgesic properties.
- 2The results reveal an inhibitory action of lappaconitine (10 μm) manifested in a slow attenuation of the orthodromic and antidromic population spike.
- 3The lappaconitine-induced inhibitory action was activity-dependent, that is, it was potentiated when frequency of electrical stimulation was increased. In contrast, washout of the neurotoxin was accelerated when stimulation frequency was decreased.
- 4The activity-dependent action of lappaconitine raised the question of whether the drug is effective in suppressing the aberrant neuronal activity that occurs during an epileptic seizure. The results obtained from experiments on epileptic hippocampal slices demonstrated a selective reduction of the later spikes in the bursts with less effect on normal neuronal activity.
- 5These data support the conclusion that lappaconitine, in addition to its antinociceptive effect, also has antiepileptic potency due to its highly activity-dependent mode of action.