Activation of vascular KCNQ (Kv7) potassium channels reverses spasmogen-induced constrictor responses in rat basilar artery
Version of Record online: 22 AUG 2011
© 2011 The Authors. British Journal of Pharmacology © 2011 The British Pharmacological Society
British Journal of Pharmacology
Volume 164, Issue 2, pages 237–249, September 2011
How to Cite
Mani, B. K., Brueggemann, L. I., Cribbs, L. L. and Byron, K. L. (2011), Activation of vascular KCNQ (Kv7) potassium channels reverses spasmogen-induced constrictor responses in rat basilar artery. British Journal of Pharmacology, 164: 237–249. doi: 10.1111/j.1476-5381.2011.01273.x
- Issue online: 22 AUG 2011
- Version of Record online: 22 AUG 2011
- Accepted manuscript online: 16 FEB 2011 11:49PM EST
- Received; 3 September 2010; Revised; 3 December 2010; Accepted; 12 January 2011
Figure S1 A. Current-voltage curves show response of 4-AP-sensitive Kv currents to the addition of 100 μM Gd3+. The currents were isolated using protocol described previously (Mackie et al., 2008). B. Normalized conductance plots of 4-AP-sensitive-Kv currents fitted by a single Boltzmann function shows positive shift in activation of the currents with the addition of Gd3+ (half maximal activation of currents is shifted by approximately 15 mV). This enabled isolation of Kv7 currents at voltages ≤−20 mV without contribution from 4-AP-sensitive Kv currents.
Figure S2 A. Current-voltage curves show that flupirtine significantly enhanced Kv7 currents at all tested voltages between -49.2 to +0.8 mV (n = 5 each, *P < 0.05, Paired Student's ‘t’ test, compared with control). XE991 (10 μM) abrogated Kv7 currents in the presence of 10 μM flupirtine (n = 5 each, #P < 0.05, Paired Student's ‘t’ test, compared with control).
Figure S3 A. Representative time course trace shows that 30 μM rofecoxib failed to dilate a basilar artery segment pre-constricted with 75 nM 5-HT. Application of 30 μM celecoxib completely reversed the serotonin-induced constriction of the same artery. B. Representative time course trace shows that 30 μM dimethyl celecoxib completely reversed the serotonin-induced constriction of basilar artery. C. Summary of the percentage dilation produced by rofecoxib and dimethyl celecoxib (n = 3 each, *P < 0.05 using one-way anova followed by post hoc Holm-Sidak test).
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