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Figure S1 A. Current-voltage curves show response of 4-AP-sensitive Kv currents to the addition of 100 μM Gd3+. The currents were isolated using protocol described previously (Mackie et al., 2008). B. Normalized conductance plots of 4-AP-sensitive-Kv currents fitted by a single Boltzmann function shows positive shift in activation of the currents with the addition of Gd3+ (half maximal activation of currents is shifted by approximately 15 mV). This enabled isolation of Kv7 currents at voltages ≤−20 mV without contribution from 4-AP-sensitive Kv currents.

Figure S2 A. Current-voltage curves show that flupirtine significantly enhanced Kv7 currents at all tested voltages between -49.2 to +0.8 mV (n = 5 each, *P < 0.05, Paired Student's ‘t’ test, compared with control). XE991 (10 μM) abrogated Kv7 currents in the presence of 10 μM flupirtine (n = 5 each, #P < 0.05, Paired Student's ‘t’ test, compared with control).

Figure S3 A. Representative time course trace shows that 30 μM rofecoxib failed to dilate a basilar artery segment pre-constricted with 75 nM 5-HT. Application of 30 μM celecoxib completely reversed the serotonin-induced constriction of the same artery. B. Representative time course trace shows that 30 μM dimethyl celecoxib completely reversed the serotonin-induced constriction of basilar artery. C. Summary of the percentage dilation produced by rofecoxib and dimethyl celecoxib (n = 3 each, *P < 0.05 using one-way anova followed by post hoc Holm-Sidak test).

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