Δ9-Tetrahydrocannabinol (Δ9-THC) attenuates mouse sperm motility and male fecundity

Authors

  • Daniel J Morgan,

    Corresponding author
    1. Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA
    2. Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA
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  • Charles H Muller,

    1. Department of Urology, University of Washington School of Medicine, Seattle, WA, USA
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  • Natalia A Murataeva,

    1. Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA
    2. Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA
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  • Brian J Davis,

    1. Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA
    2. Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA
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  • Ken Mackie

    1. Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA
    2. Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA
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Daniel J Morgan, Gill Center for Biomolecular Science, Indiana University, Bloomington, IN 47405, USA. E-mail: morgandj@indiana.edu

Abstract

BACKGROUND AND PURPOSE Numerous studies have shown that N-arachidonoylethanolamine (AEA) can inhibit sperm motility and function but the ability of cannabinoids to inhibit sperm motility is not well understood. We investigated the effects of WIN 55,212-2, a CB1 cannabinoid receptor agonist, and Δ9-tetrahydracannabinol (Δ9-THC) on the ATP levels and motility of murine sperm in vitro. In addition, the effects of acute administration of Δ9-THC on male fecundity were determined.

EXPERIMENTAL APPROACH Effects of Δ9-THC on basal sperm kinematics were determined using computer-assisted sperm analysis (CASA). Stop-motion imaging was performed to measure sperm beat frequency. The effect of Δ9-THC on sperm ATP was determined using a luciferase assay. Male fertility was determined by evaluating the size of litters sired by Δ9-THC-treated males.

KEY RESULTS Pretreatment of sperm for 15 min with 1 µM Δ9-THC reduced their basal motility and attenuated the ability of bicarbonate to stimulate flagellar beat frequency. Treatment with 5 µM WIN 55,212-2 or 10 µM Δ9-THC for 30 min reduced sperm ATP levels. In sperm lacking CB1 receptors this inhibitory effect of WIN 55,212-2 on ATP was attenuated whereas that of Δ9-THC persisted. Administration of 50 mg·kg−1Δ9-THC to male mice just before mating caused a 20% decrease in embryonic litter size.

CONCLUSIONS AND IMPLICATIONS Δ9-THC inhibits both basal and bicarbonate-stimulated sperm motility in vitro and reduces male fertility in vivo. High concentrations of WIN 55,212-2 or Δ9-THC inhibit ATP production in sperm; this effect of WIN 55,212-2 is CB1 receptor-dependent whereas that of Δ9-THC is not.

LINKED ARTICLES This article is part of a themed section on Cannabinoids in Biology and Medicine. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2012.165.issue-8. To view Part I of Cannabinoids in Biology and Medicine visit http://dx.doi.org/10.1111/bph.2011.163.issue-7

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