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Figure S1 Schild analysis of antagonism by NF449 at WT P2X1 receptors and the P2X1Cys2(3–4) chimera. The pA2 estimate for the WT receptor was ∼9.9, with a slope of 1.17 ± 0.16 (n = 6–14 for each concentration). For the P2X1Cys2(3–4) chimera the pA2 estimate was ∼6.4 with a slope of 0.84 ± 0.02 (n = 3 for each concentration).

Table S1 Properties of P2X1 receptor chimeras and mutants. Effects of the P2X1 chimeras and mutants on the peak current, rise time, decay time, ATP potency, Hill slope, NF449 and suramin sensitivity. Peak current values taken on the first application of a maximal concentration of ATP (100 or 300 μM). Rise time represents the time from 10 to 90% of the peak current and decay time represents the time 100 to 50% decay of the current. pEC50 values calculated from the individual concentration response curves. pEC50 is −log10 of the EC50 for ATP. Hill coefficient of the fitted curves is shown. pIC50 calculated from the individual inhibition curves. pIC50 is −log10 of the IC50 for the antagonist. Values are the mean ± SEM. Significant differences from WT P2X1 receptors are indicated in bold, *P < 0.05 **P < 0.01 ***P < 0.001 measured by one-way ANOVA, n = 3 to 10, ND, not determined.

Table S2 Properties of P2X2 receptor chimeras and mutants. Effects of the P2X2 chimeras and mutants on the peak current, ATP potency, Hill slope, NF449 and suramin sensitivity. Peak current values taken on the first application of a maximal concentration of ATP. pEC50 values calculated from the individual concentration response curves. pEC50 is −log10 of the EC50 for ATP. Hill coefficient of the fitted curves is shown. pIC50 calculated from the individual inhibition curves. pIC50 is −log10 of the IC50 for the antagonist. Values are the mean ± SEM. Significant differences from WT P2X2 receptors are indicated in bold, *P < 0.05 **P < 0.01 ***P < 0.001 measured by one-way ANOVA, n = 3 to 10, ND, not determined

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