Present address: Faculty of Health and Medical Sciences, Wolfson Cell Science Building, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Prostaglandin E2 induces spontaneous rhythmic activity in mouse urinary bladder independently of efferent nerves
Version of Record online: 16 DEC 2011
© 2011 The Authors. British Journal of Pharmacology © 2011 The British Pharmacological Society
British Journal of Pharmacology
Volume 165, Issue 2, pages 401–413, January 2012
How to Cite
Kobayter, S., Young, J. and Brain, K. (2012), Prostaglandin E2 induces spontaneous rhythmic activity in mouse urinary bladder independently of efferent nerves. British Journal of Pharmacology, 165: 401–413. doi: 10.1111/j.1476-5381.2011.01543.x
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- Issue online: 16 DEC 2011
- Version of Record online: 16 DEC 2011
- Accepted manuscript online: 15 JUN 2011 02:05AM EST
- Received; 26 July 2010; Revised; 19 April 2011; Accepted; 1 June 2011
Figure S1 In addition to the effects on WCTs, PGE2 appeared to increase the frequency of subcellular Ca2+ transients, including waves. Some of these subcellular Ca2+ transients also appear to be periodic. A control series of recordings and a subsequent recording from the same field of view in the presence of PGE2 (50 μM) are shown. The scale bar represents 5 μm; for display purposes this movie plays at twice the sampling rate.
Figure S2 PGE2-induced coupled WCTs in smooth muscle cells. A series of confocal images showing WCTs temporally synchronized in neighbouring cells – those shown in Figure 8A. Each frame is 50 μm across; for display purposes this movie plays at twice the sampling rate.
Figure S3 Labelling of spindle-shaped cells reminiscent of ICCs. In most experiments, only smooth muscle (sm) cells were identified; in some experimental series, spindle-shaped cells reminiscent of ICCs, Schwann cells or fibroblasts (IC?) could be identified, brightly labelled with the Ca2+ indicator. To date, such cells have been unresponsive to physiological stimuli. The field size is 100 μm.
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