Figure S1 In addition to the effects on WCTs, PGE2 appeared to increase the frequency of subcellular Ca2+ transients, including waves. Some of these subcellular Ca2+ transients also appear to be periodic. A control series of recordings and a subsequent recording from the same field of view in the presence of PGE2 (50 μM) are shown. The scale bar represents 5 μm; for display purposes this movie plays at twice the sampling rate.

Figure S2 PGE2-induced coupled WCTs in smooth muscle cells. A series of confocal images showing WCTs temporally synchronized in neighbouring cells – those shown in Figure 8A. Each frame is 50 μm across; for display purposes this movie plays at twice the sampling rate.

Figure S3 Labelling of spindle-shaped cells reminiscent of ICCs. In most experiments, only smooth muscle (sm) cells were identified; in some experimental series, spindle-shaped cells reminiscent of ICCs, Schwann cells or fibroblasts (IC?) could be identified, brightly labelled with the Ca2+ indicator. To date, such cells have been unresponsive to physiological stimuli. The field size is 100 μm.

BPH_1543_sm_FS1.mov4481KSupporting info item
BPH_1543_sm_FS2.mov1120KSupporting info item
BPH_1543_sm_FS3.pdf194KSupporting info item

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