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Figure S1 DiI-labelled liposomes are endocytosed by renal tubular epithelial cells. Twenty-four hours after injection, kidneys of C57BL/6 mice injected i.v. with (a) PBS or (b) DiI-labelled (red fluorescent) liposomes were removed and snap frozen in OCT. Sections of frozen kidney were stained with DAPI as shown (original magnification 200×). Liposomes located to renal tubular epithelial cells (Figures 1B, G = glomerulus, T = tubule).

Figure S2 DiI-labelled liposomes are endocytosed by macrophages and DC. Twenty-four hours after injection, kidneys of C57BL/6 mice injected i.v. with (a) PBS or (b) DiI-labelled (red fluorescence) liposomes were removed and digested. Isolated APC were stained with anti-CD11b FITC, anti-CD11c FITC or PE-CY7, anti-F480 PE-CY5, anti-CD8a APC, anti-B220 PE-CY5, anti-PDCA FITC, or anti-MHC APC and analysed by flow cytometry. Red fluorescent cells were gated as shown (P2) and appear black in each plot. Results are representative of three independent experiments.

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