Figure S1 Chronic mTOR inhibition alters insulin-dependent phosphorylation of Akt substrates in skeletal muscles. Wistar rats fed a standard diet were chronically administered for 3 weeks with either vehicle (CTL) or 2 mg·kg−1·day−1 Sirolimus (SIR). Western analyses were then performed on explanted skeletal muscle tissues (gastrocnemius) of insulin-stimulated rats (tissues sampled immediately at the end of the 2-DG procedure carried out under 2 h constant insulin infusion at a rate of 18 mU·kg−1·min−1) to analyse the phosphorylation of Akt potential substrates in skeletal muscles. Representative blots are derived from three different animals per group.

Table S1 Reagents and antibodies

Table S2 Primer sequences for real-time PCR

BPH_1716_sm_Figure1.tif1762KSupporting info item
BPH_1716_sm_tables.doc88KSupporting info item

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