SEARCH

SEARCH BY CITATION

Figure S1 The bar graphs reveal hypotensive and bradycardic effects of unilateral administration of different doses of renin in the nucleus tractus solitarii (NTS). Both the mean blood pressure (MBP) and heart rate (HR) effects were significantly changed by renin. *P < 0.05 versus the 0 group.

Figure S2 (A) The cardiovascular effect of injection of renin (240 fg) into the NTS after administration of the selective nNOS inhibitor, vinyl-L-NIO (600 pmol). Representative tracings demonstrate that vinyl-L-NIO did not diminish the depressor effect of renin in the NTS. Summary data (means ± SEM, n = 6) are shown in the graph (B) The cardiovascular effect of injection of renin (240 fg) into the NTS after administration of the potent nNOS inhibitor, 7-NI (66 pmol). Representative tracings demonstrate that 7-NI did not diminish the depressor effect of renin in the NTS. Summary data (means ± SEM, n = 6) are shown in the graph. (C) Immunoblot depicts the levels of P-nNOS-Ser1416 protein in the NTS. Densitometric analysis of P-nNOS-Ser1416 protein levels (means ± SEM, n = 6) before and after treatment with renin. Note that there are no significant differences in nNOS-Ser1416 phosphorylation levels in the NTS between the aCSF and renin-treated groups. (D) Immunoblot shows iNOS protein levels (means ± SEM, n = 6) in the NTS. Note that there are no significant differences in iNOS protein levels in the NTS between aCSF and the renin-treated groups.

Figure S3 (A) Immunoblots depicting the levels of P-ERK1/2-Thr202/Tyr204 protein in the NTS. There were no significant differences in ERK1/2 phosphorylation levels in the NTS between the aCSF and renin-treated groups. Densitometric analysis shows P-ERK1/2-Thr202/Tyr204 protein levels (means ± SEM, n = 6) after treatment with renin. (B) The cardiovascular effect of injection of renin (240 fg) into the NTS after administration of the MEK inhibitor, PD98059 (10 pmol). Representative tracings demonstrate that PD98059 did not diminish the depressor effect of renin in the NTS. Summary data (means ± SEM, n = 6) are shown in the graph.

Figure S4 Blood pressure (BP) after injection of renin (240 fg) into the NTS following administration of the Gβγ inhibitor, gallein (240 fmol). Representative tracings demonstrate that the depressor effect of renin was not attenuated by gallein. Summary data (means ± SEM, n = 6) are shown in the graph.

Table S1 Cardiovascular response to microinjection of renin (240 fg) into the NTS in rats before and after microinjection of the pharmacological inhibitors

FilenameFormatSizeDescription
BPH_1832_sm_Data.pdf292KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.