Figure S1 C17.2 NPCs exposed to irradiation showed a dose-dependent increase in cell death.

To evaluate cell death and viability in response to irradiation, nuclei were stained with Hoechst 33342 and PI, and visualized by fluorescence microscopy. Radiation doses were found to be dose-dependently related to numbers of PI-stained cells (dead cells). Scale bar = 100 μm.


Figure S2 Detrimental effects of WBI on NPC proliferation in the hippocampi of mice. At one day after WBI, significant dose-dependent decreases were observed in the numbers of newly generated cells. (A) Representative images showing the number of BrdU-positive cells in the dentate gyrus. (B) Numbers of newly generated cells in the SGZ significantly decreased at 24 h post-WBI. WBI substantially and dose-dependently reduced proliferating cell numbers at dose from 5 to 30 Gy. Values are the means ± SE (n = 3 mice per group). **P < 0.01 versus controls (anova using Fisher's PLSD procedure). Scale bar = 100 μm.

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