Figure S1 cAMP data showing that HEK293S cells lack endogenous responses from RAMPs, CLR or CTR. If endogenous CTR was present, the introduction of RAMP1 would have resulted in a potent CGRP response.

Figure S2 Expression of mRNA encoding CTR, CLR, RAMP1, RAMP2, RAMP3 and positive controls GAPDH in HEK293S cells by RT-PCR. Lanes 2, 4, 6, 8 and 10 are positive controls for CTR, CLR, R1, R2 and R3 primers, respectively, and show bands of the predicted size. Lanes 1, 3, 5, 7 and 9 are the paired results from mRNA prepared from HEK293S cells. No products were detected. Lane 11 shows a GAPDH housekeeping gene as a positive control for the cDNA.

Table S1 Primers used for generating FLAGhRAMP constructs.

Table S2 Summary of pEC50's for pERK1/2 responses in Cos7 cells. Data were analysed by unpaired t-tests.

Table S3 Rank order of agonist potencies observed at Δ(1–47)hCT(a), Δ(1–47)hAMY1(a), Δ(1–47)hAMY3(a) and their full-length receptors in Cos 7 cells and Δ(1–47)hCT(a), Δ(1–47)hAMY2(a) and their full-length receptors in HEK293S cells.

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