Receptor activity-modifying protein-dependent impairment of calcitonin receptor splice variant Δ(1–47)hCT(a) function
Article first published online: 16 JAN 2013
© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society
British Journal of Pharmacology
Volume 168, Issue 3, pages 644–657, February 2013
How to Cite
Qi, T., Dong, M., Watkins, H., Wootten, D., Miller, L. and Hay, D. (2013), Receptor activity-modifying protein-dependent impairment of calcitonin receptor splice variant Δ(1–47)hCT(a) function. British Journal of Pharmacology, 168: 644–657. doi: 10.1111/j.1476-5381.2012.02197.x
- Issue published online: 16 JAN 2013
- Article first published online: 16 JAN 2013
- Accepted manuscript online: 5 SEP 2012 04:33AM EST
- Manuscript Accepted: 10 AUG 2012
- Manuscript Revised: 7 AUG 2012
- Manuscript Received: 3 JUL 2012
- National Institutes of Health. Grant Number: DK46577
Figure S1 cAMP data showing that HEK293S cells lack endogenous responses from RAMPs, CLR or CTR. If endogenous CTR was present, the introduction of RAMP1 would have resulted in a potent CGRP response.
Figure S2 Expression of mRNA encoding CTR, CLR, RAMP1, RAMP2, RAMP3 and positive controls GAPDH in HEK293S cells by RT-PCR. Lanes 2, 4, 6, 8 and 10 are positive controls for CTR, CLR, R1, R2 and R3 primers, respectively, and show bands of the predicted size. Lanes 1, 3, 5, 7 and 9 are the paired results from mRNA prepared from HEK293S cells. No products were detected. Lane 11 shows a GAPDH housekeeping gene as a positive control for the cDNA.
Table S1 Primers used for generating FLAGhRAMP constructs.
Table S2 Summary of pEC50's for pERK1/2 responses in Cos7 cells. Data were analysed by unpaired t-tests.
Table S3 Rank order of agonist potencies observed at Δ(1–47)hCT(a), Δ(1–47)hAMY1(a), Δ(1–47)hAMY3(a) and their full-length receptors in Cos 7 cells and Δ(1–47)hCT(a), Δ(1–47)hAMY2(a) and their full-length receptors in HEK293S cells.
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