SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
bph2197-sup-0001-si.zip514K

Figure S1 cAMP data showing that HEK293S cells lack endogenous responses from RAMPs, CLR or CTR. If endogenous CTR was present, the introduction of RAMP1 would have resulted in a potent CGRP response.

Figure S2 Expression of mRNA encoding CTR, CLR, RAMP1, RAMP2, RAMP3 and positive controls GAPDH in HEK293S cells by RT-PCR. Lanes 2, 4, 6, 8 and 10 are positive controls for CTR, CLR, R1, R2 and R3 primers, respectively, and show bands of the predicted size. Lanes 1, 3, 5, 7 and 9 are the paired results from mRNA prepared from HEK293S cells. No products were detected. Lane 11 shows a GAPDH housekeeping gene as a positive control for the cDNA.

Table S1 Primers used for generating FLAGhRAMP constructs.

Table S2 Summary of pEC50's for pERK1/2 responses in Cos7 cells. Data were analysed by unpaired t-tests.

Table S3 Rank order of agonist potencies observed at Δ(1–47)hCT(a), Δ(1–47)hAMY1(a), Δ(1–47)hAMY3(a) and their full-length receptors in Cos 7 cells and Δ(1–47)hCT(a), Δ(1–47)hAMY2(a) and their full-length receptors in HEK293S cells.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.