These authors equally contributed to this paper.
Cross-talk between toll-like receptor 4 (TLR4) and proteinase-activated receptor 2 (PAR2) is involved in vascular function
Article first published online: 20 DEC 2012
© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society
British Journal of Pharmacology
Special Issue: Themed Section: Endothelin. Guest Editors: Anthony P Davenport and Matthias Barton
Volume 168, Issue 2, pages 411–420, January 2013
How to Cite
Bucci, M., Vellecco, V., Harrington, L., Brancaleone, V., Roviezzo, F., Mattace Raso, G., Ianaro, A., Lungarella, G., De Palma, R., Meli, R. and Cirino, G. (2013), Cross-talk between toll-like receptor 4 (TLR4) and proteinase-activated receptor 2 (PAR2) is involved in vascular function. British Journal of Pharmacology, 168: 411–420. doi: 10.1111/j.1476-5381.2012.02205.x
- Issue published online: 20 DEC 2012
- Article first published online: 20 DEC 2012
- Accepted manuscript online: 7 SEP 2012 07:03AM EST
- Manuscript Accepted: 6 AUG 2012
- Manuscript Revised: 16 JUL 2012
- Manuscript Received: 6 DEC 2011
- Ministero della Università e della Ricerca
Figure S1 (A) Western blot analysis shows a significant increase in PAR2 expression in LPS (13.6 U·kg−1) treated rats. (B) PAR2AP-induced vasodilatation was significantly increased in aortic rings harvested from LPS-treated rats (4 and 8 h after LPS) *P < 0.05 versus saline; ***P < 0.001 versus saline; n = 10 for each group. (C) Western blot analysis revealed no difference in TLR4 expression in LPS-treated compared with naive rats.
Figure S2 qRT-PCR analysis performed on kidney (A) or on aorta (B) harvested from TLR4–/– mice demonstrates that LPS causes an upregulation of PAR2 expression. (*P < 0.05 ***P < 0.001; n = 3 experiments).
Figure S3 (A) Western blot analysis showing PAR2 expression in aorta from wild-type and PAR2 KO mice. (B) Western blot analysis showing TLR4 expression in aorta from wild-type and TLR4 KO mice. Blots are representative of 3 different experiments. (C) CRC (10 μM) and RSV (10 μm) significantly inhibited PAR2AP-induced vasodilatation in aorta harvested from C57BL/10ScN mice (*** = P < 0.001 vs. vehicle; n = 3 mice for a total of 10 rings for each group).
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