Reading between the lines: molecular characterization of five widely used canine lymphoid tumour cell lines

Authors

  • E. L. Seiser,

    1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
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    • These authors contributed equally to this study.

  • R. Thomas,

    1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
    2. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
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    • These authors contributed equally to this study.

  • K. L. Richards,

    1. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    2. Division of Hematology/Oncology, University of North Carolina at Chapel Hill, NC, USA
    3. Lineberger Comprehensive Cancer Center, Chapel Hill, NC, USA
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  • M. Kathryn Kelley,

    1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
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  • P. Moore,

    1. Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA
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  • S. E. Suter,

    Corresponding author
    1. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    2. Lineberger Comprehensive Cancer Center, Chapel Hill, NC, USA
    3. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
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  • M. Breen

    Corresponding author
    1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
    2. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    3. Lineberger Comprehensive Cancer Center, Chapel Hill, NC, USA
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M. Breen, PhD, CBiol, FSB, Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. Ph: 919-513-1467; Fax: 919-513-7301; e-mail: Matthew_Breen@ncsu.edu

S. E. Suter, VMD, PhD, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. Ph: 919-513-0813; Fax: 919-513-7301; e-mail: Steven_Suter@ncsu.edu

Abstract

Molecular characterization of tumour cell lines is increasingly regarded as a prerequisite for defining their validity as models of in vivo neoplasia. We present the first comprehensive catalogue of genomic and transcriptional characteristics of five widely used canine lymphoid tumour cell lines. High-resolution microarray-based comparative genomic hybridization defined their unique profiles of genomic DNA copy number imbalance. Multicolour fluorescence in situ hybridization identified aberrant gains of MYC, KIT and FLT3 and deletions of PTEN and CDKN2 in individual cell lines, and also revealed examples of extensive structural chromosome reorganization. Gene expression profiling and RT-PCR analyses defined the relationship between genomic imbalance and transcriptional dysregulation in each cell line, clarifying their relevance as models of discrete functional pathways with biological and therapeutic significance. In combination, these data provide an extensive resource of molecular data for directing the appropriate use of these cell lines as tools for studying canine lymphoid neoplasia.

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