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VEGF and MMP-9: biomarkers for canine lymphoma

Authors

  • L. Aresu,

    Corresponding author
    1. Department of Public Health, Comparative Pathology and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Padova, Legnaro, PD, Italy
    • Correspondence address:

      Dr. L. Aresu

      Department of Public Health,

      Comparative Pathology and Veterinary Hygiene,

      Faculty of Veterinary Medicine

      University of Padova

      Viale dell'Università 16

      35020 Agripolis,Legnaro,

      PD, Italy

      e-mail: luca.aresu@unipd.it

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  • A. Aricò,

    1. Department of Public Health, Comparative Pathology and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Padova, Legnaro, PD, Italy
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  • S. Comazzi,

    1. Department of Animal Pathology, Public Health and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Milano, Milano, Italy
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  • M. E. Gelain,

    1. Department of Animal Pathology, Public Health and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Milano, Milano, Italy
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  • F. Riondato,

    1. Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, Grugliasco, TO, Italy
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  • M. Mortarino,

    1. Department of Animal Pathology, Public Health and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Milano, Milano, Italy
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  • E. Morello,

    1. Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, Grugliasco, TO, Italy
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  • D. Stefanello,

    1. Department of Veterinary Science, Faculty of Veterinary Medicine, University of Milano, Milano, Italy
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  • M. Castagnaro

    1. Department of Public Health, Comparative Pathology and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Padova, Legnaro, PD, Italy
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Abstract

Vascular endothelial growth factor (VEGF) and metalloproteinase (MMP) 2 and 9 are useful biomarkers in human lymphoma. During cancerogenesis, transforming growth factor beta (TGF-β) stimulates VEGF and MMPs production. VEGF and TGF-β plasma levels were tested by ELISA, MMP-2 and MMP-9 by gelatine zymography in 37 dogs with lymphoma, 13 of which were also monitored during chemotherapy. Ten healthy dogs served as control. Lymphoma dogs showed higher act-MMP-9 (P < 0.01) and VEGF (P < 0.05), and lower TGF-β than controls, and a positive correlation between act-MMP-9 and VEGF (P < 0.001). Act-MMP-9 and VEGF were significantly higher in T-cell lymphomas, and in stage V compared with stages III–IV disease, regardless of immunophenotype. VEGF was higher in high-grade compared with low-grade T-cell lymphomas. No correlation was found between cytokines levels at presentation and outcome. During chemotherapy, act-MMP-9 and VEGF decreased in B-cell lymphomas (P < 0.01), suggesting a possible predictive role in this group of dogs.

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