Ultrasound detection of spontaneous hepato-cellular carcinomas in X/myc bitransgenic mice
Article first published online: 26 NOV 2004
Volume 24, Issue 6, pages 651–657, December 2004
How to Cite
Mai, W., Barraud, L., Lefrancois, L., Scoazec, J.Y., Guerret, S., Vray, D., Merle, P., Vitvitski-Trepo, L., Trepo, C. and Janier, M.F. (2004), Ultrasound detection of spontaneous hepato-cellular carcinomas in X/myc bitransgenic mice. Liver International, 24: 651–657. doi: 10.1111/j.1478-3231.2004.0955.x
- Issue published online: 26 NOV 2004
- Article first published online: 26 NOV 2004
- Received 20 January 2004, accepted 19 April 2004
- hepatocellular carcinoma;
- x/myc bitransgenic model
Abstract: Aims: To evaluate trans-abdominal ultrasound for the detection of Hepatocellular carcinoma (HCC) in a bitrasgenic murine (X/myc) model using a commercially available high-frequency ultrasound unit.
Methods: Sixty-one female animals were included in this study. These animals were submitted to a single ultrasound examination of the liver under general anesthesia (isoflurane), and then euthanized. Results of ultrasound were compared with necropsy and histopathology.
Results: The lesions demonstrated a fairly consistent aspect (oval- or round-shaped, well-defined hypoechoic homogeneous lesions), and lesions as small as 2 mm were identified. For detection of hepatic nodules per mouse the sensitivity was 75%, the specificity was 100% and the accuracy was 88.5%. For detection of hepatic focal lesions per lesions the overall sensitivity was 60%, the specificity was 97%, and the accuracy was 75.9%. Contrast-enhanced harmonic ultrasound imaging did not improve the identification of the lesions in our experimental conditions.
Conclusion: High-frequency ultrasound appears to be an efficient tool allowing new possibilities to use this animal model and evaluate new therapies in longitudinal studies, which are much more powerful.