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Decreased expression of peroxisome proliferator-activated receptor α and liver fatty acid binding protein after partial hepatectomy of rats and mice


Stanko Skrtic, MD, PhD, Research Center for Endocrinology and Metabolism, Sahlgrenska University Hospital, Göteborg University, SE-413 45 Göteborg, Sweden
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Abstract: Background Aims: Marked changes in metabolism, including liver steatosis and hypoglycemia, occur after partial hepatectomy. Peroxisome proliferator-activated receptor α (PPARα) is a nuclear hormone receptor that is activated by fatty acids and involved in hepatic fatty acid metabolism and regeneration. Liver fatty acid binding protein (LFABP) is an abundant protein in liver cytosol whose expression is regulated by PPARα. It is involved in fatty acid uptake and diffusion and in PPARα signaling. The aim of this study was to investigate the expression of PPARα and LFABP during liver regeneration.

Methods: Male Sprague–Dawley rats and male C57 Bl/6 mice were subjected to 2/3 hepatectomy and LFABP and PPARα mRNA and protein levels were measured at different time points after surgery. The effect of partial hepatectomy was followed during 48 h in rats and 72 h in mice.

Results: PPARα mRNA and protein levels were decreased 26 h after hepatectomy of rats. The LFABP mRNA and protein levels paralleled those of PPARα and were also decreased 26 h after hepatectomy. In mice, the mRNA level was decreased after 36 and 72 h after hepatectomy. In this case, LFABP mRNA levels decreased more slowly after partial hepatectomy than in rats.

Conclusions: A marked decrease in PPARα expression may be important for changed gene expression, e.g. LFABP, and metabolic changes, such as hypoglycemia, during liver regeneration.