Differential modulation of interleukin 8 by interleukin 4 and interleukin 10 in HepG2 cells treated with acetaldehyde

Authors

  • Luis Enrique Gómez-Quiroz,

    1. Experimental Biology PhD Program
    2. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Raquel Paris,

    1. Liver Unit, Instituto de Malalties Digestives, Hospital Clinic i Provincial, Instituto de Investigaciones Biomédicas August Pi Suñer, Consejo Superior Investigaciones Científicas, Barcelona, Spain,
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  • José María Lluis,

    1. Liver Unit, Instituto de Malalties Digestives, Hospital Clinic i Provincial, Instituto de Investigaciones Biomédicas August Pi Suñer, Consejo Superior Investigaciones Científicas, Barcelona, Spain,
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  • Leticia Bucio,

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Verónica Souza,

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Elizabeth Hernández,

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Mario Gutiérrez,

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Mariana Santiago,

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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  • Carmen García-Ruiz,

    1. Liver Unit, Instituto de Malalties Digestives, Hospital Clinic i Provincial, Instituto de Investigaciones Biomédicas August Pi Suñer, Consejo Superior Investigaciones Científicas, Barcelona, Spain,
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  • José C. Fernández-Checa,

    1. Liver Unit, Instituto de Malalties Digestives, Hospital Clinic i Provincial, Instituto de Investigaciones Biomédicas August Pi Suñer, Consejo Superior Investigaciones Científicas, Barcelona, Spain,
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  • David Kershenobich,

    1. Departamento de Medicina Experimental, Facultad de Medicina, UNAM, México, D.F. México
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  • Ma. Concepción Gutiérrez-Ruiz

    1. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México,
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Dra. Ma. Concepción Gutiérrez-Ruiz, Departamento de Ciencias de la Salud División de Ciencias Biológicas y de la Salud Universidad Autónoma Metropolitana-Iztapalapa Apdo. Postal 55-535 México, D.F. 09340, México
e-mail: mcgr@xanum.uam.mx

Abstract

Abstract: Background/Aim: Pro-inflammatory cytokines and chemokines, such as interleukin (IL) 8, are important mediators of hepatic injury and repair following an insult. The purpose of this work was to study the regulation of IL-8 by IL-10 and IL-4 in HepG2 cells treated with acetaldehyde (Ac). Methods: HepG2 cells were pretreated with IL-10 or IL-4 before exposure to Ac, examining IL-8 expression by reverse transcription polymerase chain reaction and Western blot.

Results: Ac treatment produced an increment in IL-8 induction and secretion that was prevented by IL-4 pretreatment, while IL-10 pretreatment failed to decrease Ac-induced IL-8 production. Consistent with these findings Ac increased NF-κB and AP-1 activation that were prevented by IL-4 but not by IL-10, findings accompanied by greater IκB-α levels in IL-4 but not IL-10 pretreated cells. In contrast to the pro-inflammatory role of IL-10 in HepG2, IL-10 did not show any change in the activation of NF-κB by Ac in WRL-68 cells, a human fetal hepatic cell line. Moreover, IL-10 did not induce the degradation of IκB-α in cellular extract from rat primary cultured cells.

Conclusions: While the present findings demonstrate the anti-inflammatory role of IL-4 in preventing the expression of IL-8 by Ac, the regulation of chemokines by anti-inflammatory cytokines is complex and depends on the cellular lineage.

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