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Keywords:

  • mitosis;
  • nuclear factor-κB;
  • proliferating cell nuclear antigen;
  • transcription factor

Abstract: Aims/Background: Tumor necrosis factor-α (TNF-α) is known as a proinflammatory cytokine that has been implicated as a contributing factor in a number of disease processes. TNF-α also influences liver repair following hepatotoxic damage, and regeneration following partial hepatectomy (PH). The aim of this study was to assess the mechanism by which TNF-α influences liver cell apoptosis and regeneration following PH in TNF-α-deficient (TNF-α−/−) mice.

Methods: PH was performed in wild mice and TNF-α−/− mice.

Results: In both groups, serum alanine aminotransferase and serum total bilirubin levels comparably peaked at 6 and 48 h after PH, respectively. No differences were observed in hepatocyte proliferation, as determined by mitotic and the proliferating cell nuclear antigen labeling indices, between TNF-α+/+ and TNF-α−/− mice. Few terminal deoxynucleotidyl transferase nick end-labeling-positive hepatocytes were seen in either type of mice. Nuclear factor-κB DNA binding activity in the remaining liver of TNF-α−/− mice after PH was similar to that of control mice. Ribonuclease protection assay showed that transforming growth factor β1 mRNA was up-regulated comparably in the livers of the two groups, and that other cytokines were hardly seen in either. Interleukin-6/ signal transducer and activator of transcription-3-dependent pathway was not affected in TNF-α−/− mice.

Conclusions: These findings suggest that TNF-α has little influence on liver regeneration and liver cell apoptosis after PH in mice.