Upregulation of Glypican-3 expression in hepatocellular carcinoma but downregulation in cholangiocarcinoma indicates its differential diagnosis value in primary liver cancers

Authors

  • Xiao-Bo Man,

    1. International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institue, The Second Military Medical University, Shanghai, China,
    Search for more papers by this author
  • Liang Tang,

    1. International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institue, The Second Military Medical University, Shanghai, China,
    Search for more papers by this author
  • Bao-Hua Zhang,

    1. Department of Biliary Surgery,
    Search for more papers by this author
  • Shen-Jing Li,

    1. International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institue, The Second Military Medical University, Shanghai, China,
    Search for more papers by this author
  • Xiu-Hua Qiu,

    1. International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institue, The Second Military Medical University, Shanghai, China,
    Search for more papers by this author
  • Meng-Chao Wu,

    1. Department of Clinical Surgery, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, China
    Search for more papers by this author
  • Hong-Yang Wang

    1. International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institue, The Second Military Medical University, Shanghai, China,
    Search for more papers by this author

Prof. Hong-Yang Wang, International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, The Second Military Medical University, Shanghai 200438, China.
Tel: +86 21 25070856
Fax: +86 21 65566851
e-mail: hywangk@online.sh.cn

Abstract

Abstract: Background/Aim: Expression alteration of Glypican-3 (GPC3) is associated with several malignancies and has been identified as an overexpressed gene in hepatocellular carcinoma (HCC). In this study GPC3 expression in intrahepatic chanlangiocarcinoma (ICC), gallbladder cancer and HCC was quantitatively detected.

Methods: Real-time quantitative reverse transcription polymerase chain reaction was used to detect the expression level of GPC3.

Results: GPC3 expression was elevated more than two-fold in HCC compared with adjacent tissue in 90 of 100 HCC cases. The average expression level of GPC3 was significantly higher in HCC than that in adjacent liver tissues (P<0.0001). Only in four of 21 ICC cases GPC3 expression was upregulated more than two-fold in tumor tissues. GPC3 expression was downregulated in gallbladder cancer in 12 of 13 cases and the average expression level was significantly lower than that in normal gallbladder tissues (P<0.05).

Conclusion: The different expression patterns of GPC3 in HCC and ICC suggested that it might play a different role in theses tumors and could serve as a biomarker for differential diagnosis of HCC and ICC.

Ancillary