Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application


  • Prior Publication: Friedenberg M, Chung CY, Miller LS, Fleszler F, Banson F,Thomas RM, Friedenberg F. Automated Color Segmentation Simplifies Quantification of Liver Fibrosis. AGA Poster Session. Digestive Disease Week, New Orleans, LA, May 2004.

Frank Friedenberg, MD, Temple University Hospital, 8th Floor Parkinson Pavilion, 3401 N. Broad Street, Philadelphia, PA 19140, USA.
Tel: +1 215 707 3431
Fax: +1 215 707 2684


Abstract: Introduction: Semiquantitative evaluation of liver specimens is considered the standard method for measuring fibrosis; however, these systems lack the precision of a quantitative technique.

Methods: We developed an image analysis application (FibroXact) that automates and simplifies color segmentation. Trichrome slides were scanned and the program was used to evaluate each pixel based upon hue, lightness, and saturation values. Percent fibrosis was automatically calculated after thresholding.

Results: Linear regression demonstrated a strong relationship between the FibroXact result and the semiquantitative result (P<0.001).

Conclusion: We have developed an image analysis application to quantify liver fibrosis. Correlation between our results and a standard semiquantitative system was demonstrated.