Different growth capacity between infant and adult mouse hepatocytes in vitro correlates to the cyclin D1 level without relation to oxidative DNA damage
Article first published online: 30 JUN 2005
Volume 25, Issue 5, pages 1036–1043, October 2005
How to Cite
Imamura, E., Yamamoto, M., Miyakoshi, M., Honmo, S., Ozaki, A., Yoshie, M., Tamakawa, S., Yaginuma, Y., Kasai, S. and Ogawa, K. (2005), Different growth capacity between infant and adult mouse hepatocytes in vitro correlates to the cyclin D1 level without relation to oxidative DNA damage. Liver International, 25: 1036–1043. doi: 10.1111/j.1478-3231.2005.1125.x
- Issue published online: 14 JUL 2005
- Article first published online: 30 JUN 2005
- Received 21 September 2004, accepted 1 February 2005
- cyclin D1;
- primary hepatocytes;
Abstract: Background: Proliferating capacity of hepatocytes is rapidly decreased during growth into maturity, but its exact reason(s) are not well known.
Methods: Hepatocytes isolated from infant (10–14 days old) and adult (10–13 months old) B6C3F1 mice were cultivated in the medium containing epidermal growth factor and insulin. Proliferative capacity, apoptosis, morphological changes, cell cycle proteins and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were compared between the two hepatocyte populations.
Results: Although adult hepatocytes rapidly underwent cellular crisis characterized by extended morphology and multiple nuclei without proliferation, infant hepatocytes could proliferate with less crisis. Cyclin D1 was much more abundant in the infant than adult cells, but there was no difference according to the expression of cdk4, cdk2, cyclin E and cdk inhibitors (p16Ink4 (p16), p21Cip1/Waf1 (p21) and p27Kip1 (p27)). 8-OHdG became high soon after cultivation, while it rapidly went down after day 2 both in the infant and adult cells.
Conclusions: The high growth capacity of infant hepatocytes in vitro was dependent on the cyclin D1 level, but there was no relation to 8-OHdG.