Distribution of hepatitis B virus genotypes among patients with chronic infection
Version of Record online: 11 MAY 2006
Volume 26, Issue 6, pages 636–642, August 2006
How to Cite
Caldas Ribeiro, N. R., Soares Campos, G., Dias Angelo, A. L., Lorens Braga, E., Santana, N., Mesquita Soares Gomes, M., Rebello Pinho, J. R., Andrade De Carvalho, W., Costa Lyra, L. G. and Castro Lyra, A. (2006), Distribution of hepatitis B virus genotypes among patients with chronic infection. Liver International, 26: 636–642. doi: 10.1111/j.1478-3231.2006.01280.x
- Issue online: 27 JUN 2006
- Version of Record online: 11 MAY 2006
- Received 3 October 2005,accepted 19 March 2006
- chronic hepatitis B;
- HBV genotype;
- hepatitis B virus;
- inactive chronic carriers;
Abstract: Hepatitis B virus (HBV) can be classified into at least eight genotypes, A–H. We evaluated the distribution HBV genotypes among patients with chronic infection.
Methods: We consecutively evaluated adult patients with chronic HBV infection from Salvador, Brazil. Patients were classified according to HBV infection chronic phases based on HBV-DNA levels and presence of serum HBV markers. HBV-DNA was qualitatively and quantitatively detected in serum by polymerised chain reaction (PCR). Isolates were genotyped by comparison of amino acid mutations and phylogenetic analysis.
Results: One-hundred and fourteen patients were evaluated. HBV-DNA was positive in 96 samples. HBV genotype was done in 76. Mean age was 36±11.3. In 61 of 76 cases subjects were classified as inactive HBsAg carriers. Their mean HBV serum level was 1760 copies/ml and 53 of 61 were infected with HBV genotype A, seven with HBV genotype F and one with genotype B. Twelve of the 76 patients had detectable hepatitis B e-antigen (HBeAg) in serum. Ten were infected with HBV genotype A and two with genotype F; most had increased alanine aminotransferase and high HBV-DNA levels. Three patients were in the immunotolerant phase, two were infected with HBV genotype A and one with genotype F. HBV subtyping showed subtypes adw2 and adw4.
Conclusions: HBV genotype A adw2 and genotype F adw4 were the most prevalent isolates found. We could not find differences in genotype distribution according to HBV clinical phases and DNA levels. We did not detect HBV genotype D in contrast to a previous study in our center with acute hepatitis B. All inactive HBsAg carriers had low HBV-DNA levels.